Antimicrobial effect coordinative compounds of copper with 4-(dimethylphenyl)-thiosemicarbazones of 2-phormylpyridine

Abstract

Background: This study is referring to a new group of coordinative compounds of copper with 4-(dimethylphenyl)-thiosemicarbazones 2-phormylpyridine, which posses a high antibacterial activity against gram-positive and gram-negative microorganisms and which present new directions in the elaborations of antibacterial preparations. Material and methods: The antimicrobial activity has been studied in vitro in liquid nutritive media [peptone broth, 2%, pH 7.0] by means of using the method of successive dilutions. The substances were dissolved in DMSO in concentration of 10 ml, the subsequent dilutions were prepared in peptone broth. The reference strains Staphylococcus aureus, Bacillus cereus, Escherihia coli, Salmonella abony, and Shigela sonnei were used as reference cultures. Determination of minimal inhibitory concentration and minimal bactericidal concentration was performed by using the standard method described in the literature. Results: The results of the study demonstrate the antimicrobial activity of the compounds, coordinative compounds I-VI that exhibit antimicrobial activity against gram-positive microorganisms 320-520 times higher than furacilina (prototype) and 16-8 times higher than their analogous structures. Conclusions: Coordinative compounds of copper 4-(dimethylphenyl)-2-phormylpyridine thiosemicarbazones newly synthesized posses high bactericidal and bacteriostatic activity against a wide spectrum of microorganisms such as gram-positive and gram-negative bacteria. The experimentally obtained data demonstrate that coordinative compounds I-VI manifest antimicrobial activity against gram-positive microorganism of 320-520 times higher than furacilin (prototype) and 16-8 times higher than their structural analogue. The bacteriostatic and bactericidal activity of I-IV compounds against Escherichia coli and Salmonella abony is equivalent with the furacilin one, but against Shigella sonnei exceeds the prototypes activity by 320-8 times. The identified properties of nominated compounds present interest because of expanding arsenal of antimicrobial remedies and can be used for combating the strains of microorganisms which are resistant to traditional drugs.