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Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12710/11121
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dc.contributor.authorGoecke, T.
dc.contributor.authorCebotari, S.
dc.contributor.authorTudorache, I.
dc.contributor.authorHaverich, A.
dc.contributor.authorHilfiker, A.
dc.date.accessioned2020-07-08T05:16:13Z
dc.date.available2020-07-08T05:16:13Z
dc.date.issued2016
dc.identifier.citationGOECKE, T., CEBOTARI, S., TUDORACHE, I., Haverich, A., HILFIKER., A. Removal of xenoantigenic glycosylation patterns from porcine pulmonary heart valve matrices is dependent of the applied decellularization method. In: MedEspera: the 6th Internat. Medical Congress for Students and Young Doctors: abstract book. Chișinău: S. n., 2016, p. 124-125.en_US
dc.identifier.isbn978-9975-3028-3-8.
dc.identifier.urihttp://repository.usmf.md/handle/20.500.12710/11121
dc.descriptionDepartment of Cardiac-, Thoracic-, Transplantation and Vascular Surgery, Hannover Medical School, Hannover Germany and Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Hannover Medical School, Hannover, Germany, The 6th International Medical Congress for Students and Young Doctors, May 12-14, 2016en_US
dc.description.abstractIntroduction: Matrix guided tissue regeneration (GTR) based on allogeneic decellularized matrices has been shown as an overall convincing method for heart valve replacement. Nevertheless, a substantial donor shortage prevents an unlimited clinical application of human GTR-valves. Utilization of porcine decellularized heart valve matrices could offer a possible solution for overcoming this considerable limitation. In the past, implantation of xenogeneic valve tissues considered to be acellularinto human recipients, however, mostly lead to severe immune responses usually ending up into graft rejection. This study addresses the question whether potential xenoantigenic glycosylation of extracellular matrix components, like the major xenoantigen α-Gal, which served as model epitope for this study, can be removed by adjusted decellularization procedures. Materials and methods: Fresh porcine pulmonary heart valve conduits were decellularized by application of different detergent- and enzyme-based decellularization protocols. Subsequent cleavage of remaining matrix-related α-Gal epitopes was performed by enzymatic deglycosylation treatment on matrix samples of each decellularization group. Resulting tissues, mainly composed from insoluble extracellular matrix proteins, were afterwards divided into the relevant sections pulmonary artery wall specimens and pulmonary valve leaflets, frozen in liquid nitrogen, minced and finally solubilized by protease digestion. Evaluation of thus prepared solutions regarding to α-Gal contents was finally performed using a novel designed lectin-based immunoblot technique. Discussion results: Sole decellularization lead to significant removal of α-Gal, substantial varying in strong dependency to applied protocols between 30 to 50% compared to α-Gal contents of porcine native control tissues. An additional decrease of residual α-Gal in a range of another 15 to 30% was achievable by additional α-Galactosidase treatment. Combining decellularization and subsequent enzymatic digestion resulted in reductions of matrix related α-Gal contents down to levels, which could be measured for respective pulmonary valve tissues of α-Gal-KnockOut pigs. Conclusion: Residual xenoantigenic carbohydrates are detectable on insoluble matrix components of porcine pulmonary heart valves, substantially varying dependent on applied decellularization protocols. Combined with glycolytic digestions, remaining glycosylation contents are reducible to background levels. Impacts of these novel insights have to be evaluated in further in vitro as well as in vivo studies.en_US
dc.language.isoenen_US
dc.publisherMedEsperaen_US
dc.subjectXenotransplantationen_US
dc.subjectDecellularizationen_US
dc.subjectDeglycosylationen_US
dc.subjectHeart valvesen_US
dc.subjectTissue Enigneeringen_US
dc.titleRemoval of xenoantigenic glycosylation patterns from porcine pulmonary heart valve matrices is dependent of the applied decellularization methoden_US
dc.typeArticleen_US
Appears in Collections:MedEspera 2016



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