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Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12710/11763
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dc.contributor.authorCazacu, Eugeniu
dc.contributor.authorVataman, Vladimir
dc.contributor.authorPretula, Ruslan
dc.date.accessioned2020-09-24T05:53:17Z
dc.date.available2020-09-24T05:53:17Z
dc.date.issued2020
dc.identifier.citationCAZACU, Eugeniu, VATAMAN, Vladimir, PRETULA, Ruslan. Epithelio-mesenchymal transition process in the pathogenesis of extragenital endometriosis. In: MedEspera: the 8th Internat. Medical Congress for Students and Young Doctors: abstract book. Chișinău: S. n., 2020, p. 246-247.en_US
dc.identifier.urihttps://medespera.asr.md/wp-content/uploads/ABSTRACT-BOOK.pdf
dc.identifier.urihttp://repository.usmf.md/handle/20.500.12710/11763
dc.descriptionDepartment of Morphopathology, Nicolae Testemitanu State University of Medicine and Pharmacy, Chisinau, Republic of Moldova, The 8th International Medical Congress for Students and Young Doctors, September 24-26, 2020en_US
dc.description.abstractIntroduction. Epithelial – mesenchymal transition (EMT) endows cells with migratory and invasive proprieties, a prerequisite for the establishment of endometriotic lesions. The role EMT might play in the pathophysiology of endometriosis is still unknow. Therefore, we examined four markers for EMT in endometrium and endometriosis: E – cadherin + Vimentin, double reactions and simple reactions Twist and N – cadherin. Aim of the study. Immunohistochemical assessment of the invasiveness potential of extragenital endometriosis lesions by investigating some of the specific markers (β-catenin / vimentin panel) of the epithelio-mesenchymal transition process (EMT), a process by which epithelial cells lose their polarity and contact with the polarity and contact invasive. Materials and methods. During a period of five years (2012 – 2017) we analyzed 41 cases of extragenital endometriosis: appendix 5, colon 7, intestine 8, anterior abdominal wall after caesarean operation- 10, inguinal hernia – 6, umbilical hernia- 4, perineal region- 1. The material was processed according to the classic histological technique by inclusion in paraffin. The 3 μm sections obtained were stained with Hematoxylin – Eosin and Masson's trichrome stains. Another sections were dewaxed, rehydrated and processed for immunohistochemistry using as primary antibodies monoclonal antibodies Vimentin and mouse monoclonal antibody N – cadtherin, E – cadherin, Twist. Results. Immunohistochemically, we aimed to change the immunophenotype from epithelial to mesenchyme in extragenital endometriosis by analyzing the most important markers of the transition process. In endometriosis and endometrium E – cadherin, Vimentin, N – cadherin and Twist were expressed on protein level. Investigation of E – cadherin / Vimentin coexpression revealed a decrease in E – cadherin reactivity at the site of invasion of gastrointestinal endometriosis with an increase in reactivity to Vimentin together with the increase of the invasion pattern and the increase of the stage of the disease respectively. Twist transcription factor immunoexpression revealed a highly positive expression on the mesenchymal lineage, proving involvement of this transcriptional factor in the invasion process of endometriosis. N – cadherin was positive in the endometrial glands, showing their differentiation into a mesenchymal phenotype and their migratory potential. Conclusions. The results of our study confirm involvement of the epithelial – mesenchymal transition process in the pathogenesis of extragenital endometriosis lesions, on the one hand, and they certify their invasive potential in these localizations, on the other hand.en_US
dc.language.isoenen_US
dc.publisherMedEsperaen_US
dc.subjectendometriosisen_US
dc.subjectextragenitalen_US
dc.subjecttransition prossesen_US
dc.titleEpithelio-mesenchymal transition process in the pathogenesis of extragenital endometriosisen_US
dc.typeArticleen_US
Appears in Collections:MedEspera 2020

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