USMF logo

Institutional Repository in Medical Sciences
of Nicolae Testemitanu State University of Medicine and Pharmacy
of the Republic of Moldova
(IRMS – Nicolae Testemitanu SUMPh)

Biblioteca Stiintifica Medicala
DSpace

University homepage  |  Library homepage

 
 
Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12710/11894
Full metadata record
DC FieldValueLanguage
dc.contributor.authorRacoviță, Stela-
dc.date.accessioned2020-10-02T06:49:32Z-
dc.date.available2020-10-02T06:49:32Z-
dc.date.issued2020-
dc.identifier.citationRACOVIȚĂ, Stela. Defects in spermatogenesis of men with Y chromosome microdeletions. In: MedEspera: the 8th Internat. Medical Congress for Students and Young Doctors: abstract book. Chișinău: S. n., 2020, p. 289-290.en_US
dc.identifier.urihttps://medespera.asr.md/wp-content/uploads/ABSTRACT-BOOK.pdf-
dc.identifier.urihttp://repository.usmf.md/handle/20.500.12710/11894-
dc.descriptionDepartment of Molecular Biology and Human Genetics, Nicolae Testemitanu State University of Medicine and Pharmacy, Chisinau, Republic of Moldova, The 8th International Medical Congress for Students and Young Doctors, September 24-26, 2020en_US
dc.description.abstractIntroduction. Male infertility has multiple etiology, most commonly caused by spermatogenesis disorders, clinically manifested by oligo/azoospermia. Until recently, Y microdeletion had little clinical significance since men with a deletion were considered unable to reproduce. However, by utilizing of Intracytoplasmic sperm injection (ICSI) and Testicular sperm extraction (TESE) it is now possible for oligo/azoospermic men with Y microdeletion to father children. Aim of the study. To analyze the type of defect in spermatogenesis associated with specific Y deletions found in our IVF program, for prevention the transmission of these deletions through ICSI to offspring. Materials and methods. A group of 46 infertile men were investigated during genetic counseling among infertile couples referred for ART treatment. Criteria for including patients were fulfilled if they presented with oligo/azoospermia, raised or normal levels of FSH, LH and testosterone. Genomic DNA was isolated and used to analyze AZF microdeletions by PCR. The regions and sequence-tagged sites of AZFa (SY86, SY84), AZFb (SY127, SY134), and AZFc (SY254, SY255) were sequenced by multiplex PCR. Five non-obstructive azoospermic men had Y chromosomal microdeletions. All five Y-microdeleted men underwent microsurgical observation of testicular architecture and quantitative histology of spermatogenesis in a strip of testicular tissue. The results were compared with the different type of Y microdeletion. Results. Deletions of Y chromosome were seen in the AZFc regions of 2 patients, deleted markers were sY254 and sY255. In both men with AZFc deletions, the histological defects were variable, but no sperm were found. In only one case the defect of Sertoli cell-only syndrome (SCOS) in patient with microdeletions in each region of AZFa-sY84, sY86; AZFbsY127, sY134; AZFc-sY254, sY255 was present. One patient with deletion of AZFb (SY127, SY134) had spermatogenetic maturation arrest. In all men with AZF microdeletions of the Y chromosome, we found severe spermatogenic defects: however, we also did not find, in all of them, mature sperm sufficient for ICSI. The patients were advised to use sperm from the donor for ICSI and IVF. Conclusions. This study highlights for all couples with the diagnosis of male infertility with oligo/azoospermia the need of genetic testing and counseling prior to employment of assisted reproduction techniques. This is important for providing a firm diagnosis and fertility treatment to couples with infertility and for prevention of the transmission of AZFc deletions through ICSI to offspring.en_US
dc.language.isoenen_US
dc.publisherMedEsperaen_US
dc.subjectmale infertilityen_US
dc.subjectPCRen_US
dc.subjectdeletionen_US
dc.subjectAZF regionen_US
dc.titleDefects in spermatogenesis of men with Y chromosome microdeletionsen_US
dc.typeArticleen_US
Appears in Collections:MedEspera 2020

Files in This Item:
File Description SizeFormat 
Racovita_Stela.pdf444.29 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2013  Duraspace - Feedback