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Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12710/12164
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dc.contributor.authorUshkalov, Valerii
dc.contributor.authorSalmanov, Aidyn
dc.contributor.authorKalachniuk, Liliia
dc.contributor.authorVishovan, Yuriy
dc.contributor.authorBoianovskiy, Sergiy
dc.contributor.authorUshkalov, Artem
dc.contributor.authorGranate, Andry
dc.contributor.authorHuwiage, Goma M.
dc.contributor.authorKepple, Oleksandra
dc.date.accessioned2020-10-14T17:54:52Z
dc.date.available2020-10-14T17:54:52Z
dc.date.issued2020
dc.identifier.citationUSHKALOV, Valerii, SALMANOV, Aidyn, KALACHNIUK, Liliia, et al. The influence of cultivation temperature on some phenotypic traits of Yersinia pseudotuberculosis. In: One Health & Risk Management. 2020, vol. 1, no 2, pp. 34-40. ISSN 2587-3458. DOI: 10.38045/ohrm.2020.1.14
dc.identifier.issn2587-3458
dc.identifier.issn2587-3466
dc.identifier.uriDOI: 10.38045/ohrm.2020.1.14
dc.identifier.urihttp://repository.usmf.md/handle/20.500.12710/12164
dc.identifier.urihttps://journal.ohrm.bba.md/index.php/journal-ohrm-bba-md/article/view/70/48
dc.descriptionNational University of Life and Environmental Sciences of Ukraine, Ukrainian Laboratory of Quality and Safety of Agricultural Products, Shupyk National Medical Academy of Postgraduate Education, Ukrainian National Committee of Infection Control (UCIC) and Antimicrobial Resistance (UCAST), Main administration of state service of Ukraine on food safety and consumer protection in Kharkov region, High Institute of Medical Science & Technology, Algarabolli, Libyaen_US
dc.description.abstractIntroduction. Some causative agents of sapro-zoonotic infections can multiply in the external environment (water reservoirs, soils, plants), as well as in animals, including microorganisms of the genus Yersinia. Material and methods. Isolation and identification of Y. pseudotuberculosis was carried out in accordance with the instructions on “Epidemiology, laboratory diagnosis of yersiniosis, organization and conduct of preventive and anti-epidemiological measures”. Antibiotic sensitivity was performed via the disc diffusion method in accordance with EUCAST and national guidelines. Biofilm formation was tested using the spectrophotometric assay. Results. It was established that the studied cultures showed a decrease in the level of saccharolytic activity during cultivation at a temperature of +37°C in comparison with the results obtained at 25°C, changes in sensitivity to antibiotics depending on the temperature of cultivation were revealed. It was established that Y. pseudotuberculosis cultures were able to form denser (λ 570) biofilms when cultured at 25°C, in comparison with biofilms formed at 37°C. Conclusions. Biological characteristics of the studied Y. pseudotuberculosis isolates (changes in the saccharolytic activity, the level of sensitivity to antibiotics and the formation of biofilms were revealed) depends on the cultivation conditions.en_US
dc.description.abstractIntroducere. Unii agenți cauzali ai sapronozelor se pot multiplica atât în mediul extern (rezervoare de apă, sol, plante), cât și în organismul animalelor, la astfel de agenți patogeni atribuindu-se și bacteriile din genul Yesinia. Material și metode. Izolarea și identificarea Y. pseudotuberculosis a fost efectuată în conformitate cu instrucțiunea „Epidemiologia, diagnosticul de laborator al yersiniozei, organizarea și desfășurarea măsurilor preventive și antiepidemice”. Sensibilitatea la antibiotice a fost efectuată prin metoda difuzimetrică, în conformitate cu EUCAST și gidul național. Capacitatea de a forma biofilme, cât și densitatea lor, au fost determinate prin metoda spectrofotometrică după densitatea optică. Rezultate. Culturile studiate au demonstrat o scădere a nivelului activităţii zaharolitice, în timpul cultivării la temperatura de +37°C, în comparație cu rezultatele obținute la 25°C, totodată înregistrându-se şi diferenţe ale sensibilității la antibiotice în funcție de temperatura de cultivare. S-a mai stabilit că culturile de Y. pseudotuberculosis au fost capabile să formeze biofilme mai dense (λ 570) la temperatura de 25°C, decât cele formate la 37°C. Concluzii. S-a stabilit că, manifestarea proprietăților biologice ale izolatelor studiate de Y. pseudotuberculosis (modificări ale activității zaharolitice, nivelul de sensibilitate la antibiotice și formarea de biofilme) depinde de condițiile de cultivare.rom
dc.language.isoenen_US
dc.publisherAsociația de Biosiguranță și Biosecuritate din Republica Moldovaen_US
dc.relation.ispartofOne Health & Risk Management
dc.subjectYersinia pseudotuberculosisen_US
dc.subjectcultivation temperatureen_US
dc.subjectphenotypic traitsen_US
dc.subjectantibioticsen_US
dc.subjectresistanceen_US
dc.subjectsensitivityen_US
dc.subjectbiofilmsen_US
dc.subject.ddcUDC: 616.98:578.834.1(478)
dc.titleThe influence of cultivation temperature on some phenotypic traits of Yersinia pseudotuberculosisen_US
dc.title.alternativeInfluența temperaturii de cultivare asupra unor proprietăți fenotipice ale Yersinia pseudotuberculosisen_US
dc.typeArticleen_US
Appears in Collections:One Health & Risk Management Vol. 1 No 2, 2020



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