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Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12710/14761
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dc.contributor.authorPalii, Lucian-
dc.contributor.authorHotineanu, Adrian-
dc.contributor.authorBarbacar, Nicolae-
dc.contributor.authorTimiş, Tudor-
dc.contributor.authorBendelic, Valentin-
dc.date.accessioned2021-01-06T12:09:14Z-
dc.date.available2021-01-06T12:09:14Z-
dc.date.issued2020-
dc.identifier.citationPALII, Lucian, HOTINEANU, Adrian, BARBACAR, Nicolae, TIMIŞ, Tudor, BENDELIC, Valentin. Minisatellite instability of hMLH1, hMSH2 genes in hereditary colorectal adenomas (Lynch sindrome) = Instabilitatea minisatelitică (IMS) a genelor hMLH1, hMSH2 în adenomii colorectali ereditari (sindromul Lynch). In: Congresul consacrat aniversării a 75-a de la fondarea USMF „Nicolae Testemițanu”, 21-23 octombrie 2020: Abstract book. Chișinău: [s. n.], 2020, p. 433.en_US
dc.identifier.urihttps://stiinta.usmf.md/sites/default/files/inline-files/Abstract%20Book.%20CULEGERE%20DE%20REZUMATE%20.pdf-
dc.identifier.urihttp://repository.usmf.md/handle/20.500.12710/14761-
dc.description.abstractBackground. In the light of the progress achieved in the area of improving the methods of recombined DNA technology, today it has become possible to diagnose a genetic disease, including the Lynch Syndrome as a form of epithelial colorectal neoplasia (ECRN) at the level of genes (hMLH1, hMSH2). Objective of the study. Analysis of the results of genetic research, at the molecular level of the hMLH1, hMSH2 genes involved in triggering the mechanism of tumorogenesis. Material and Methods. During the years 2012-2019, a group of 55 patients were observed and treated, of which 19 patients were diagnosed with IMS. In the identification of the genetic associations between the polymorphic DNA spectra and the clinical manifestations of the studied precancerous (tumorogenic) diseases, the PCR technique of the isolated DNA samples from the investigated patients was used. Results. Following the RT-PCR reaction based on RNA isolated from the biological material, 30 cases (54.5%) with negative expression were found, a positive result of grade I (+) was determined in 14 cases (26, 2%) and grade II (++) in 10 cases (19%). The molecular-genetic origin of NECR was confirmed and the value of the polymorphic appearance of the hMLH1, hMSH2 genes, their level and frequency of expression in tumor tissues was determined. Conclusion. The RT-PCR technique used in the experimental study contributes to increasing the efficiency of the medical-genetic consultation and to the eradication of colorectal cancer already at the early stages of development. Introducere. Evoluția obscură și manifestările clinice fruste ale neoplaziei epiteliale colorectale (NECR) necesită în prezent o implimentare cât mai largă a investigaţiilor genetico-moleculare în procesul de diagnostic. Scopul lucrării. Analiza rezultatelor cercetărilor genetice, la nivel molecular, ale genelor hMLH1, hMSH2 implicate în declanşarea mecanismului de tumorogeneză. Material și Metode. În perioada anilor 2012-2019, au fost observaţi şi trataţi un lot de 55 de bolnavi, dintre care la 19 pacienţi s-a depistat IMS. În identificarea asocierilor genetice dintre spectrele polimorfe de ADN şi manifestările clinice ale maladiilor precanceroase (tumorogene) studiate s-a utilizat tehnica PCR a eşantioanelor de ADN izolate de la pacienţii cercetaţi. Rezultate. În urma reacţiei RT-PCR, pe baza ARN-lui izolat din materialul biologic, s-au constatat 30 de cazuri (54,5%) cu expresie negativă, rezultat pozitiv de gradul I(+) a fost determinat în 14 cazuri (26,2%) şi gradul II(++) în 10 cazuri (19%). A fost confirmată originea NECR la nivel molecular-genetic și determinată valoarea aspectului polimorf al genelor hMLH1, hMSH2, nivelul şi frecvenţa lor de expresie în ţesuturile tumorale. Concluzii. Tehnica RT-PCR utilizată în studiul experimental contribuie la creşterea eficienţei consultului medico-genetic şi la eradicarea cancerului colorectal deja la etapele incipiente de dezvoltare.en_US
dc.publisherUniversitatea de Stat de Medicină şi Farmacie "Nicolae Testemiţanu" din Republica Moldovaen_US
dc.subjecthereditary colorectal adenomasen_US
dc.subjectgenes (hMLH1, hMSH2)en_US
dc.titleMinisatellite instability of hMLH1, hMSH2 genes in hereditary colorectal adenomas (Lynch sindrome)en_US
dc.title.alternativeInstabilitatea minisatelitică (IMS) a genelor hMLH1, hMSH2 în adenomii colorectali ereditari (sindromul Lynch)en_US
dc.typeOtheren_US
Appears in Collections:Congresul consacrat aniversării a 75-a de la fondarea USMF „Nicolae Testemițanu”, 21-23 octombrie 2020: Abstract book

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