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Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12710/11794
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dc.contributor.authorFulga, Ala
dc.contributor.authorPantea, Valeriana
dc.date.accessioned2020-09-29T14:32:24Z
dc.date.available2020-09-29T14:32:24Z
dc.date.issued2020
dc.identifier.citationFULGA, Ala, PANTEA, Valeriana. Antioxidant activity of Taraxacum officinale. In: MedEspera: the 8th Internat. Medical Congress for Students and Young Doctors: abstract book. Chișinău: S. n., 2020, p. 267-268.en_US
dc.identifier.urihttps://medespera.asr.md/wp-content/uploads/ABSTRACT-BOOK.pdf
dc.identifier.urihttp://repository.usmf.md/handle/20.500.12710/11794
dc.descriptionDepartment of Biochemistry and Clinical Biochemistry, Nicolae Testemitanu State University of Medicine and Pharmacy, Chisinau, Republic of Moldova, The 8th International Medical Congress for Students and Young Doctors, September 24-26, 2020en_US
dc.description.abstractIntroduction. Taraxacum officinale, considered a weed in many crops around the world, is regarded as a fully nontoxic and entirely edible plant. The chemical composition of plant (flowers, leaves, roots and latex) has been studied extensively due to its biological actions: antioxidant, anti-inflammatory, anti-carcinogenic, anti-hyperglycaemic, anti-thrombotic, antimicrobial and antiviral. Aim of the study. To establish the optimal conditions for the extraction of the bioactive compounds from the Taraxacum officinale leaves and their characterization (study of the physical-chemical and biological properties). Materials and methods. The process of extraction from the investigated vegetal material has been realized by using the maceration during 24 hours. The extraction has been realized at a room temperature in three consecutive steps for each method using as a solvents: 80%, 50%, and 20% of ethanol. The antioxidant properties of the obtained extracts was investigated by the ABTS (2,2-azinobis-(3-ethylbenzothiazoline)-6-sulfonic acid) method described by Re et al., with some modifications. The results were expressed as percent inhibition of the ABTS radical. Trolox (6-hydroxy-2,5,7,8-tetramethychroman-2-carboxylic acid) and Rutin (Quercetin-3- rutinoside hydrate; Sigma-Aldrich) was used as antioxidant standard. Results. The highest antioxidant properties in the leaves of Taraxacum officinale showed the extraction with 80% EtOH, at concentrations of 1.25, 0.63 and 0.31 μg/ml, the inhibition percentage in the ABTS test was: 93%, 77% and 48%, respectively. The lower inhibition activity was observed with 50% EtOH extract at concentrations of 4.69, 2.34 and 1.17 μg/ml, the inhibition rate was 95%, 94% and 94%. A similar ABTS radical inhibition activity also showed the 20% EtOH extracts, which at concentrations of 4.38, 2.19 and 1.1 μg/ml, showed an inhibition rate of 95%, 94% and, respectively, 81%. The biologically active compounds extracted from the leaves of Taraxacum officinale more effectively capture the ABTS radical compared to the reference substances - Trolox and Rutin. Thus, Trolox and Rutin at concentrations of 187.5, 93.75 and 46.9 μg/ml, showed an inhibition rate in the ABTS test equal to 40%, 20%, 13% and, respectively, 40%, 24% and 17%. The biologically active compounds present in the leaves of Taraxacum officinale can act as free radical capture agents, thus participating in the prevention of DNA adduct formation and in the prevention of mutagenesis and carcinogenesis and may also exert chemopreventive effects. Conclusions. In this research, we developed an environmentally sustainable procedure for determining the antioxidant properties of Taraxacum officinale leaf extracts. We applied this procedure to select the most efficient method for extracting bioactive molecules with the highest antioxidant activity in the ABTS free radical capture assay. This method is fast, nontoxic, low cost and environmentally sustainable and due to its efficiency, it can be used in various biomedical applications.en_US
dc.language.isoenen_US
dc.publisherMedEsperaen_US
dc.subjectABTS free radical capture assayen_US
dc.subjectTaraxacum officinaleen_US
dc.subjectextraction of biologically active compoundsen_US
dc.titleAntioxidant activity of Taraxacum officinaleen_US
dc.typeArticleen_US
Appears in Collections:MedEspera 2020

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