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Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12710/11830
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dc.contributor.authorCernat, Victor
dc.contributor.authorStope, Mathias
dc.contributor.authorKönsgen, Dominique
dc.contributor.authorDiesing, Karoline
dc.contributor.authorWiegank, Luise
dc.contributor.authorMustea, Alexander
dc.date.accessioned2020-09-30T14:11:31Z
dc.date.available2020-09-30T14:11:31Z
dc.date.issued2016
dc.identifier.citationCERNAT, Victor, STOPE, Mathias, KÖNSGEN, Dominique, [et al]. Expression of the MIR-1 molecule in patients with uterine leiomyosarcoma. In: MedEspera: the 6th Internat. Medical Congress for Students and Young Doctors: abstract book. Chișinău: S. n., 2016, pp. 246-247.en_US
dc.identifier.isbn978-9975-3028-3-8.
dc.identifier.urihttp://repository.usmf.md/handle/20.500.12710/11830
dc.descriptionDepartment of Surgery, University Medicine Greifswald, Ferdinand-Sauerbruch-Strasse, 17475 Greifswald, Germany, The 6th International Medical Congress for Students and Young Doctorsen_US
dc.description.abstractBackground: The uterine leiomyosarcoma represents the most frequent malignant gynecologic mesenchymal tumor that often develops distant metastases. The diagnosis of these tumors is nowadays still a challenge and the direct implication of the small non-coding RNAs (MicroRNAs ) in gene expression, tumor initiation and tumor progression has already been revealed in scientific studies. Because the aberrant microRNA (miRNA) expression patterns show a diagnostic value as tumor markers, we aimed to identify the gene expression level of miRNA-1 (miR-1) and the protein targets in uterine leiomyosarcoma. Methods: Using the specific cell line - SK-UT-1 with similar biological characteristics of the uterine leiomyosarcoma tissue, in comparison to ovarian carcinoma cell lines: OVCAR-3, TOV-21 and SK-OV-3, and cell lines of mouse heart-muscle (HL-1), we were able to perform real time PCRs and RNA-Isolation arrays, transient and stabile transfection programs with lipofectamine reagents. Tissue samples of uterine leiomyosarcoma and healthy uterus were again analyzed by means of transfection and isolation arrays. The electrophoresis using protein targets of the miR-1 (p38 and ERK 1/2 widely expressed protein kinase intracellular signaling molecules and involved in functions including the regulation of meiosis, mitosis, und postmitotic functions) was also integrated. Results: The analysis of the SK-UT-1 cell line have shown significant differences in comparison to the other studied cell lines, respectively a reduced expression of the miR-1 molecules. The same results were observed in the process of transfection and electrophoresis of the human tissues, where the lowest expression of the miR-1 was evidenced in the uterine leiomyosarcomas. The specific protein targets of miR-1 have shown positive Western Blot signals. Conclusions: The miR-1 non coding molecules may improve our understanding of disease development, progression and gene expression of the uterine leiomyosarcoma. Further prospective translational studies in order to evaluate miR-1 as a prognostic factor are needed. Key words: MIR-1, leiomyosarcoma, Western Blot.en_US
dc.language.isoenen_US
dc.publisherMedEsperaen_US
dc.subjectMIR-1en_US
dc.subjectleiomyosarcomaen_US
dc.subjectWestern Bloten_US
dc.titleExpression of the MIR-1 molecule in patients with uterine leiomyosarcomaen_US
dc.typeArticleen_US
Appears in Collections:MedEspera 2016

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