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Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12710/12894
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dc.contributor.authorUncu, Andrei
dc.date.accessioned2020-11-11T14:28:34Z
dc.date.available2020-11-11T14:28:34Z
dc.date.issued2020-10
dc.identifier.urihttps://stiinta.usmf.md/ro/manifestari-stiintifice/zilele-universitatii
dc.identifier.urihttp://repository.usmf.md/handle/20.500.12710/12894
dc.descriptionScientific Center for Drug Research Nicolae Testemitanu State University of Medicine and Pharmacy of Republic of Moldova, Congresul consacrat aniversării a 75-a de la fondarea Universității de Stat de Medicină și Farmacie „Nicolae Testemițanu” din Republica Moldova, Ziua internațională a științei pentru pace și dezvoltareen_US
dc.description.abstractINTRODUCTION. Propylthiohinothiadiazole is a thiadiazole derivative with pronounced antimycobacterial action. Substance is researched in order to develop methods of analysis and standardization, analytical documents regulating quality for use as an active principle in the development of pharmaceutical forms. The purpose. Elaboration of the work technique and validation of the HPLC method for dosing propylthiohinothiadiazole. MATERIAL AND METHODS. 3 series of propylthiohinothiadiazole, internal reference standard of 2-(propylthio)-5H-[1,3,4]- thiadiazole[2,3-b]-quinazolin-5-one substance with concentration 99,98%, chromatographic system Shimadzu LC-20AD HPLC with UV-VIS detector, acetonitrile and dimethylsulfoxide of purity grade “pro HPLC analysis” (Sigma Aldrih and Merck), bidistilled purified water. RESULTS. Linearity is proved for concentrations of 5-30 µg/ml, the linear regression equation is y=36472x44580, R²=0.9929. The limit of detection is 0,729 µg/ml and the limit of quantification is 2,210 µg/ml. It was established that the method is accurate (mean recovery values at 80%, 100% and 120% concentration levels were close to 100%. The accuracy of the method was expressed by repeatability and intermediate accuracy. The variation of the chromatographic conditions established that the method is robust. For all validation parameters, RSD was less than 1. CONCLUSIONS. The validation results show that the developed HPLC method is simple, fast, accurate and reproducible.en_US
dc.language.isoenen_US
dc.publisherUniversitatea de Stat de Medicină şi Farmacie "Nicolae Testemiţanu" din Republica Moldovaen_US
dc.subjectHPLCen_US
dc.subjectpropylthiohinothiadiazoleen_US
dc.subjectdosingen_US
dc.subjectvalidationen_US
dc.titleDevelopment and validation of the high-pressure liquid chromatography method for propyltiohinotiadiazol dosageen_US
dc.typeOtheren_US
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