Морфогистохимическая реакция надпочечников потомства интактных и спленэктомированных крыс на введение канцерогена

Abstract

Abstract Background: One of the major problems in medicine is the increase in cancer incidence, which leads to deterioration of the environment, the impact of different mutagenic factors, increased stress load. Recently, it is recognized that the risk of cancer increases significantly on the background of immunodeficiency. Also, there were many works devoted to the “hormonal carcinogenesis” and hormonal status on the background of cancer. Material and methods: The experiments were carried out on the progeny of intact and splenectomized females (80 white male infant rats) treated with the carcinogen 1,2-dimethylhydrazine. We used luminescent-histochemical methods for the detection of structures containing biogenic amines, cytospektroflyuorimetry – for their quantitative assessment, the definition of the ratio of biogenic amines to assess the functional state of the cells, stain sections with hematoxylin and eosin and immunohistochemical methods using monoclonal antibodies – markers of cell APUD series. Results: It is found that removal of the spleen in female rats, and administration of the carcinogen to their progeny leads to significant morphological changes in the adrenal glands in the offspring. This is reflected in a decrease in the area of the adrenal medulla, a slight increase in the width of the cortex, the disorganization of the secretion of biogenic amines, increasing the number of synaptophysin-positive and neuron-positive cells. Conсlusions: Installed changes in the bioamins containing and neuroendocrine cells of the adrenal glands in the offspring of splenectomized and intact rats on the background of offspring carcinogen administration indicates the involvement of these cells in the body’s response to carcinogenesis. Research has shown that, given the innate immune changes are more pronounced.