Technologies for in vitro cultivation of skin fibroblasts for patients with chronic ulcers treatment

Abstract

Background: As many factors lead to chronic lower leg ulceration, an interdisciplinary approach to the systematic assessment of the patient is required, in order to ascertain the pathogenesis, definitive diagnosis, and optimal treatment. A number of researchers propose to use in ulcers treatment non senescent fibroblasts. By donating additional cells to the wound environment releasing extra growth factors and reversing the antiproliferative activity of chronic wound exudates can be achieved. Material and methods: The main stages of fibroblasts’ cultivation are: obtaining of skin biopsy, donors’ serologic control, preparing culture flasks, separation of epidermis and dermis, proper cultivation, cells bacteriological control and application. Results: The final cell density in primary culture was 4,5*104 cells/cm2 . It’s demonstrated that tissue-engineered human dermal fibroblasts can replace the dermis and provide essential stimulatory growth factors for wound healing. Conclusions: The overall safety and lack of rejection reactions combined with the efficacy encourage the use of HFDD in addition to good wound care practices