DC Field | Value | Language |
dc.contributor.author | Cobzac, Vitalie | - |
dc.contributor.author | Verestiuc, Liliana | - |
dc.contributor.author | Jian, Mariana | - |
dc.contributor.author | Nacu, Viorel | - |
dc.date.accessioned | 2022-01-27T10:26:27Z | - |
dc.date.available | 2022-01-27T10:26:27Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | COBZAC, Vitalie, VERESTIUC, Liliana, JIAN, Mariana, NACU, Viorel. Chondrocytes isolation from hyaline cartilage by continuous monitoring method. In: The Moldovan Medical Journal. 2021, vol. 64, no 6, pp. 13-19. ISSN 2537-6381. https://doi.org/10.52418/moldovan-med-j.64-6.21.03 | en_US |
dc.identifier.issn | 2537-6373 | - |
dc.identifier.issn | 2537-6381 | - |
dc.identifier.uri | http://moldmedjournal.md/wp-content/uploads/2021/12/Moldovan-Med-J-December-2021-Vol-64-No-6.pdf | - |
dc.identifier.uri | https://doi.org/10.52418/moldovan-med-j.64-6.21.03 | - |
dc.identifier.uri | http://repository.usmf.md/handle/20.500.12710/19720 | - |
dc.description.abstract | Background: Articular cartilage has poor regenerative capacities. Numerous cartilage repair techniques are known, including implantation of autologous
chondrocytes.
Material and methods: From 18 rabbits pieces of cartilage were harvested from femoral condyle. Minced cartilage was treated with 0.25% trypsin-EDTA.
In the 1st group (n=9) the cartilage was digested with 0.6% collagenase in 15 ml tubes by shaking in incubator at 37°C, 5%CO2. In the 2nd group (n=9)
digestion was performed in 25cm2 cell culture flasks placed on the lateral side, monitoring the process under a microscope after 120 minutes. The isolated
cells were cultured to a 80-90% confluence. The chondrocytes were identified using histochemical staining after culturing for 16 days in overconfluence.
Results: Chondrocytes isolation in the 1st group lasted a fixed 360 minutes, in the 2nd group – 140±10 minutes. In the 1st group were isolated 9.2x104±3.1x104
chondrocytes with a viability of 85.36±16.41%, but in the 2nd group – 1.6x105±3.4x104 chondrocytes with a viability of 98.09±3.85%. The mean period of
cell culture in the 1st group was 15±2 days, in the 2nd group – 11±3 days. In first passage of the 1st group were obtained – 1.2x106 ±4.3x105 chondrocytes
and in the 2nd group – 2.92x106 ±3.6x105 chondrocytes. The secreted extracellular matrix by chondrocytes was stained specifically for cartilaginous tissue.
Conclusions: The method used for chondrocytes isolation has a direct impact on the number of isolated cells, their viability, but also upon the culture
period and the number of cells obtained during the first passage. | en_US |
dc.language.iso | en | en_US |
dc.publisher | The Scientific Medical Association of the Republic of Moldova | en_US |
dc.relation.ispartof | The Moldovan Medical Journal | en_US |
dc.subject | cartilage | en_US |
dc.subject | chondrocytes isolation | en_US |
dc.subject | continuous monitoring | en_US |
dc.subject.ddc | UDC: 616.72-018.3-089.844-092.9 | en_US |
dc.title | Chondrocytes isolation from hyaline cartilage by continuous monitoring method | en_US |
dc.type | Article | en_US |
Appears in Collections: | The Moldovan Medical Journal. Vol. 64, No 6, December 2021
|