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Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12710/19720
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dc.contributor.authorCobzac, Vitalie-
dc.contributor.authorVerestiuc, Liliana-
dc.contributor.authorJian, Mariana-
dc.contributor.authorNacu, Viorel-
dc.date.accessioned2022-01-27T10:26:27Z-
dc.date.available2022-01-27T10:26:27Z-
dc.date.issued2021-
dc.identifier.citationCOBZAC, Vitalie, VERESTIUC, Liliana, JIAN, Mariana, NACU, Viorel. Chondrocytes isolation from hyaline cartilage by continuous monitoring method. In: The Moldovan Medical Journal. 2021, vol. 64, no 6, pp. 13-19. ISSN 2537-6381. https://doi.org/10.52418/moldovan-med-j.64-6.21.03en_US
dc.identifier.issn2537-6373-
dc.identifier.issn2537-6381-
dc.identifier.urihttp://moldmedjournal.md/wp-content/uploads/2021/12/Moldovan-Med-J-December-2021-Vol-64-No-6.pdf-
dc.identifier.urihttps://doi.org/10.52418/moldovan-med-j.64-6.21.03-
dc.identifier.urihttp://repository.usmf.md/handle/20.500.12710/19720-
dc.description.abstractBackground: Articular cartilage has poor regenerative capacities. Numerous cartilage repair techniques are known, including implantation of autologous chondrocytes. Material and methods: From 18 rabbits pieces of cartilage were harvested from femoral condyle. Minced cartilage was treated with 0.25% trypsin-EDTA. In the 1st group (n=9) the cartilage was digested with 0.6% collagenase in 15 ml tubes by shaking in incubator at 37°C, 5%CO2. In the 2nd group (n=9) digestion was performed in 25cm2 cell culture flasks placed on the lateral side, monitoring the process under a microscope after 120 minutes. The isolated cells were cultured to a 80-90% confluence. The chondrocytes were identified using histochemical staining after culturing for 16 days in overconfluence. Results: Chondrocytes isolation in the 1st group lasted a fixed 360 minutes, in the 2nd group – 140±10 minutes. In the 1st group were isolated 9.2x104±3.1x104 chondrocytes with a viability of 85.36±16.41%, but in the 2nd group – 1.6x105±3.4x104 chondrocytes with a viability of 98.09±3.85%. The mean period of cell culture in the 1st group was 15±2 days, in the 2nd group – 11±3 days. In first passage of the 1st group were obtained – 1.2x106 ±4.3x105 chondrocytes and in the 2nd group – 2.92x106 ±3.6x105 chondrocytes. The secreted extracellular matrix by chondrocytes was stained specifically for cartilaginous tissue. Conclusions: The method used for chondrocytes isolation has a direct impact on the number of isolated cells, their viability, but also upon the culture period and the number of cells obtained during the first passage.en_US
dc.language.isoenen_US
dc.publisherThe Scientific Medical Association of the Republic of Moldovaen_US
dc.relation.ispartofThe Moldovan Medical Journalen_US
dc.subjectcartilageen_US
dc.subjectchondrocytes isolationen_US
dc.subjectcontinuous monitoringen_US
dc.subject.ddcUDC: 616.72-018.3-089.844-092.9en_US
dc.titleChondrocytes isolation from hyaline cartilage by continuous monitoring methoden_US
dc.typeArticleen_US
Appears in Collections:The Moldovan Medical Journal. Vol. 64, No 6, December 2021



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