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Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12710/19936
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dc.contributor.authorChinedum, Nchege-
dc.date.accessioned2022-02-03T11:20:22Z-
dc.date.available2022-02-03T11:20:22Z-
dc.date.issued2012-
dc.identifier.citationCHINEDUM, Nchege. Iron oxide nanoparticles and lipid peroxidation in acute blood loss. In: MedEspera: the 4th Internat. Medical Congress for Students and Young Doctors: abstract book. Chișinău: S. n., 2012, pp. 241-242.en_US
dc.identifier.urihttp://repository.usmf.md/handle/20.500.12710/19936-
dc.description.abstractIntroduction: In many countries around the world the researchers study the iron oxide nanoparticles because of their unique super magnetic properties and an opportunity for biodegradation in the organism as well as of wide spread and cheapness of this biometal. There are more and more preparations based on the supermagnetic nanoparticles. They are used as contrast agents for a magnetic resonant tomography. They also are applied in oncology for the treatment of malignant tumors and in hematology for the therapy of ferrous-deficit anemia. It is known that nanoparticles of iron oxides have an increased chemical reactance. The authors described that inhalation of iron oxide nanoparticles is accompanied by the induction of active forms of oxygen in the lung cells. However, the data on iron nanoparticles’ influence on oxidative-reductive homeostasis are limited and have inconsistent character. Aims and objectives: The research purpose is to study the influence of iron oxide nanoparticles on lipid peroxidation and superoxide dismutase (SOD) activity in red blood cells under the conditions of acute blood loss. Methods and results: Experiments were carried out in 15 albino male Wistar rats with body weight of 180-200g. Blood loss was designed by the extraction of 25% of circulating blood from the heart under the general anesthesia inhalation. Ultrasmall supermagnetic nanoparticles of iron oxide (II, III), so called magnetite, were obtained by the method of electronic-radiation technology in the Paton Electric Welding Institute of the National Academy of Sciences of Ukraine. They are in the form of powder containing magnetite nanoparticles with a size of 8-16 nm. Suspension of these nanoparticles was prepared ex tempore and administered to the animals intraperitoneally in a dose of 1.35 mg of iron/kg immediately after the blood loss. According to the results of laser spectroscopy in such liquid 99.9% of particles have the size of 50 nm. Mass of these nanoparticles is 46% from iron oxides mass. Other fraction is represented by the particles with a size of 830 nm. SI. 8. 3 hours after that the contents of the products reacting with thiobarbituric acid (TBA-reactants) and the activity of SOD by the inhibition of epinephrine auto-oxidation were determined. The data were processed statistically by the standard programs Microsoft Excel. SI. 9. It is shown, that acute blood loss is characterized by the increase of TBA-reactants’ concentration in red blood cells up to 11.2 extinction units per milliliter in comparison with 3.2 extinction units per milliliter of blood in intact animals. The activity of SOD is 52.6% and essentially does not differ from the control. After the administration of iron oxide nanoparticles the TBA-reactants’ level is equal 7.2 extinction units per milliliter that is in 1.6 times less than in blood loss without pharmacological correction. SOD activity in this group is 46.4% that is authentically lower as compared to blood loss without nanoparticles administration. The received results testify that acute blood loss in the early period of its compensation is accompanied by amplified formation of lipid peroxidation intermediates on a background of normal SOD’s activity. The iron oxide nanoparticles reduce the expressiveness of oxidative stress that can be connected to fast improvement of hematological parameters and restoration of hemoglobin level which plays the important role in maintenance of oxidative balance in red blood cells. Conclusion: Thus, ultrasmall super magnetic nanoparticles of iron oxide (II, III) as 1.35mg of iron/ kg of body weight administered parenterally in acute blood loss can inhibit lipid peroxidation in erythrocytes. Probably, the reducing of oxidative stress in the given situation is connected with fast restoration of hemoglobin level, but the mechanism of this action demands further studying.en_US
dc.language.isoenen_US
dc.publisherState Medical and Pharmaceutical University Nicolae Testemitanu, Medical Students and Residents Association, Scientific Association of Students and Young Doctorsen_US
dc.relation.ispartofMedEspera: The 4th International Medical Congress for Students and Young Doctors, May 17-19, 2012, Chisinau, Republic of Moldovaen_US
dc.titleIron oxide nanoparticles and lipid peroxidation in acute blood lossen_US
dc.typeOtheren_US
Appears in Collections:MedEspera 2012

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