The influence of the pulsed electromagnetic fields on the proliferation and morphology of mesenchymal stem cells

Abstract

Stimulation of cell division is the source of physiological recovery that provides the most reliable perspective in tissue engineering. A non-invasive and accessible method of amplifying the process of cell division is using electromagnetic fields. Our purpose was to analyze the pulsed electromagnetic fields capacity to influence the cellular proliferation in vitro. For this purpose, were used cell cultures of mesenchymal stem cells, derived from 14 days aviary embryos. Cells were subjected to a quasi-rectangular pulsed electromagnetic field with duration of 300ps, a frequency of 7.5 Hz, 2hours each day for 7 days. The results indicate a rise with 25% of the number of cells subjected to the magnetic field, and this report was not influenced by the cell density. The cell morphology showed no difference between groups. These results suggest the possibility of using low frequency pulsed electromagnetic fields in tissue engineering with the purposes to accelerate mesenchymal stem cell division, which can be applied in bone regeneration therapy.