Genotoxicity testing of generic pesticides glyphosate in fluctuation Ames assay

Abstract

Determination of potential genotoxic effects within generic pesticide studies is an obligatory requirement for justifying their safety usage and evaluation of potential risks. In our research center, we successfully conduct the recommended standard mutagenicity test battery in comlience with GLP, which includes gene mutation tests in bacteria in vitro (fluctuation Ames assay OECD 471) and gene mutation tests in mammalian cells in vivo and in vitro (micronucleus assay OECD 475, 487 and metaphase chromosomal aberration assay OECD 474). The aim m of the study was to research the potential mutagenic effects of generic pesticides glyphosate 95.2% (Gly1), glyphosate 95.6% (Gly2), glyphosate 95.0% (Gly3) in screening fluctuation Ames assay using Sal nella typhimurium strains TA98 and TA100 with and without metabolic activation, preincubation in the suspension was 90 min. Selection of concentrations were based on preliminary experiment in pre-screening assay which was performed before the main test. In the absence of cytotoxicity and precipitation in preliminary experiment the following concentrations (5; 1; 0.2; 0.04; 0.008; 0.0016 mg/ml ) were defined. As a result: obtained experimental data of positive and negative controls were ranged with own historical control. Validated XL template was used to calculate the results. Our results showed statistically significant absence of the mutagenic effect of generic pesticides glyphosates Gly1, Gly2, Gly3 in fluctuation Ames assay.