Supravegerea epidemiologică şi de laborator a cazurilor de paralizie acută în menţinerea statutului Republicii Moldova ca ţară liberă de poliomielită

Abstract

In 2002 the European Regional Certification Commission of the World Health Organization declared Europe a zone free of poliomyelitis caused by the wild virus. The postcertification program [4] provides identification, informing, epidemiological and laboratory investigation of each case of acute flaccid paralysis (AFP) with the subsequent establishing of the final diagnosis to globally complete the eradication of polio. From 2008 to 2012 in the Republic of Moldova 45 cases of the AFP in children were registred, from which 92 feces samples were collected and studied. All the identified poliomyelitis viruses had a vaccination nature. In this work the results of the monitoring of epidemiological and laboratory cases of AFP, including the people who were contacting those ones having this disease in the last 5 years (2008-2012), have been discribed. The isolation of the viral strains and their identification in the reaction of neutralization has been held in the cultures of the cells RD and L-20B received from WHO using standard specific immune serums against polio- and enteroviroses (Bilthoven, Netherlands) in the national laboratory of poliomyelitis and enteroviroses, which is a part of the European network of laboratories for the diagnosis of these diseases (the laboratory is accredited annually by the profile experts of WHO).