http://repository.usmf.md:80/xmlui/handle/20.500.12710/883 2026-09-10T10:39:16Z 2026-09-10T10:39:16Z Balan, Greta Sofronie, Olga Cojocari, Daniela Zariciuc, Elena http://repository.usmf.md:80/xmlui/handle/20.500.12710/33575 2026-09-04T10:52:56Z 2026-01-01T00:00:00Z

Guide Microbiological specimen management: Collection, transport and storage Balan, Greta; Sofronie, Olga; Cojocari, Daniela; Zariciuc, Elena

2026-01-01T00:00:00Z Furculița, Daniel Maniuc, Mihail Danilov, Lucian Ababii, Polina http://repository.usmf.md:80/xmlui/handle/20.500.12710/33424 2026-07-01T09:15:29Z 2026-01-01T00:00:00Z

Postoperative autologous peripheral blood mononuclear cell therapy in the rehabilitation of children with chronic hypertrophic rhinitis: a prospective comparative study Furculița, Daniel; Maniuc, Mihail; Danilov, Lucian; Ababii, Polina Chronic hypertrophic rhinitis in children is a common pathology associated with inferior turbinate hypertrophy, persistent nasal obstruction, and impaired quality of life. Surgical interventions to reduce turbinate volume, such as diode laser and bipolar cautery, are widely used, but postoperative inflammation and mucosal regeneration can be prolonged. Autologous cell therapy represents a promising regenerative approach to accelerate tissue healing. Objective – to evaluate the effectiveness of postoperative autologous mononuclear cell therapy in the rehabilitation of children with chronic hypertrophic rhinitis following surgical treatment. Methods. The study was prospective, interventional, and comparative, conducted on a group of 40 children aged 7 to 17 years diagnosed with chronic hypertrophic rhinitis. Patients were divided into four groups (n = 10/group): diode laser (DL), diode laser + cell therapy (DL+CT), bipolar cautery (BC), and bipolar cautery + cell therapy (BC+CT). Cell therapy consisted of the postoperative local administration of a suspension of autologous mononuclear cells obtained from peripheral blood. The evaluation included the NOSE symptom score, rhinomanometry, acoustic rhinometry and endoscopic examination. The follow-up period was 24 months. Results. All groups showed postoperative clinical improvement. The cell therapy groups demonstrated a significantly faster and more stable reduction in symptoms, with a decrease in the NOSE score to 14.0 ± 4.0 in the DL+CT group at 24 months, compared to 30.0 ± 6.0 in DL and 36.0 ± 7.0 in BC. Nasal resistance decreased more sharply in the cell therapy groups, and the incidence of persistent edema, crusting and relapses was lower. Conclusions. Autologous cell therapy applied postoperatively accelerates the regeneration of the nasal mucosa and improves functional outcomes in children with chronic hypertrophic rhinitis, with maximum efficiency in combination with diode laser.

2026-01-01T00:00:00Z Burlac, Veronica Burduniuc, Olga Colac, Svetlana Iaconi, Oana-Simina Bucov, Victoria Lupu, Marina Iliev, A.-M. http://repository.usmf.md:80/xmlui/handle/20.500.12710/33411 2026-06-26T13:09:35Z 2025-01-01T00:00:00Z

Metagenomics sequencing in infection diagnosis: clinical applications Burlac, Veronica; Burduniuc, Olga; Colac, Svetlana; Iaconi, Oana-Simina; Bucov, Victoria; Lupu, Marina; Iliev, A.-M. By performing a literature review, to identify categories of patients in whom metagenomics sequencing has demonstrated potential clinical relevance in the diagnosis of infections, highlighting the practical applications, benefits and limitations of this method in the context of different clinical scenarios for further adaptation of clinical and laboratory diagnostic protocols. The study is descriptive and analytical. A literature review was conducted, using literature from open access databases. English language papers relevant to the proposed research topic published in the last 5 years were selected according to the inclusion criteria.Metagenomics sequencing (mNGS) is an innovative technology that has revolutionized traditional clinical diagnostics on multiple levels. It can be used for the simultaneous identification of multiple pathogens such as bacteria, viruses, fungi and parasites in clinical samples. The application of metagenomics would bring considerable benefits even in cases of rare infections with unknown etiology or pathogens that are difficult to culture. This study demonstrates how this new technology can be used for meaningful clinical diagnostics in microbiology. Patients with lower respiratory tract infections represent a category of patients for whom the use of mNGS brings several benefits, this technology has an overall higher sensitivity for pathogen detection compared to the culture method and is a necessary complement to conventional microbiologic tests. Sepsis is a major public health challenge and the use of mNGS for this category of patients can shorten the total hospitalization time and reduce mortality. Metagenomics sequencing technology is also useful for patients with central nervous system infections, the use of mNSG for those patients has a higher detection rate but the highest diagnostic yield is observed when combining mNGS with conventional methods. Metagenomics offers significant benefits in the detection of pathogens also in patients with deep cervical space abscesses, with a superior detection rate and sensitivity, allowing early initiation of antimicrobial therapy, improved prognosis and reduced consumption of medical resources. Metagenomics provides a complete, rapid and accurate identification of pathogens, significantly improving treatment, mainly when traditional methods are insufficient. Based on the studies presented in this article we can conclude that the application of metagenomics for patients with lower respiratory tract infections, sepsis, central nervous system infections and cervical deep space infections has a very high potential to reduce morbidity, mortality and health care costs.

2025-01-01T00:00:00Z Colac, Svetlana Sîtnic, Victor Burduniuc, Olga http://repository.usmf.md:80/xmlui/handle/20.500.12710/33410 2026-06-26T11:19:39Z 2024-01-01T00:00:00Z

Genomic surveillance of wastewater for SARS-CoV-2 detection in the Republic of Moldova Colac, Svetlana; Sîtnic, Victor; Burduniuc, Olga This research study explored the diversity and prevalence of SARS-CoV-2 genetic lineages in wastewater samples collected from the Chişinău wastewater treatment plant in the Republic of Moldova between September and November 2024. A total of 23 samples were analysed using Illumina sequencing technology. Although the overall quality of the sequencing data was suboptimal due to limited genomic coverage, the analysis identified 32 genetic lineages based on the Pango Lineages classification. The most common variants detected included B.1.177.66, B.1.1.243, and B.1.160.32. The study emphasises the importance of optimising the RNA extraction process from wastewater to improve genome coverage and data accuracy. These findings provide a foundation for future investigations involving sequencing genetic material obtained from environmental samples, suggesting the applicability of wastewater surveillance to other types of pathogens or assessing the resistome through metagenomic technology.; În cadrul prezentului studiu a fost evaluată diversitatea și abundența liniilor genetice ale virusului SARSCoV-2 în probe de ape uzate colectate la stația de epurare din Chișinău, Republica Moldova în perioada septembrie - noiembrie 2024. Au fost analizate 23 probe de ape reziduale utilizând secvențierea prin tehnologia Illumina. Deși calitatea generală a datelor de secvențiere a fost suboptimală, acoperirea genomică fiind redusă, studiul a permis identificarea a 32 linii genetice conform clasificării Pango Lineages. Printre cele mai abundente variante s-au numărat B.1.177.66, B.1.1.243 și B.1.160.32. În scopul îmbunătățirii acoperirii genomice și acurateții rezultatelor, în lucrare este subliniată importanța optimizării etapei de extracție a ARNului din ape uzate. Studiul oferă premise pentru viitoare investigații privind secvențierea materialului genetic obținut din probe de mediu, sugerând astfel aplicabilitatea monitorizării apelor uzate la alte tipuri de patogeni sau la evaluarea rezistomului prin tehnnologia metagenomică.

2024-01-01T00:00:00Z