http://repository.usmf.md:80/xmlui/handle/20.500.12710/254 2026-04-28T08:57:11Z http://repository.usmf.md:80/xmlui/handle/20.500.12710/33207 Performanța și proprietățile materialelor bioactive în tratamentele endodontice Ababii, Lucia; Marcu, Diana Introducere: Evoluția materialelor endodontice a contribuit semnificativ la dezvoltarea endodonției moderne. Succesul tratamentului depinde în mare măsură de proprietățile biologice și fizico-chimice ale materialelor aplicate. În acest context, materialele bioactive au devenit tot mai frecvent utilizate în practica endodontică, datorită biocompatibilității și capacității lor de a interacționa cu țesuturile dentare. Scopul lucrării: Evaluarea proprietăților materialelor bioactive utilizate în endodonție, prin analiza datelor din literatura de specialitate, în vederea evidențierii rolului acestora în tratamentul endodontic. Materiale și metode: Studiul dat a fost realizat prin analiza și sinteza literaturii de specialitate PubMed, Google Scholar și Biblioteca Națională a Republicii Moldova. Au fost analizate proprietățile materialelor bioactive utilizate în endodonție, precum MTA, Biodentine și BioRoot RCS, urmărindu-se proprietăți precum biocompatibilitatea, bioactivitatea, timpul de priză și efectul antimicrobian. Rezultate: Sinteza literaturii de specialitate evidențiază proprietățile materialelor bioactive utilizate în endodonție, caracterizate prin biocompatibilitate ridicată, demonstrată prin testul MTT, cu o viabilitate celulară de aproximativ 91,2% pentru BioRoot RCS și 87,5% pentru MTA și Biodentine. Literatura raportează bioactivitatea asociată eliberării ionilor de calciu și capacitatea de a susține procesele de mineralizare. În ceea ce privește timpul de priză, Biodentine prezintă valori mai reduse (12 min),comparativ cu MTA (2–3 ore). Rezultatele analizei indică eficacitate antimicrobiană de peste 90% pentru materialele evaluate. Concluzii: Materialele bioactive reprezintă o componentă importantă în endodonția modernă, datorită proprietăților lor biologice și fizico-chimice. Caracteristicile acestor materiale, precum interacțiunea cu țesuturile dentare și comportamentul lor biologic, contribuie la îmbunătățirea performanței tratamentelor endodontice. Diferențele existente între materiale sunt determinate de compoziția și proprietățile specifice, aspecte care pot influența alegerea lor în practica clinică.; Background: The evolution of endodontic materials has significantly contributed to the development of modern endodontics. The success of endodontic treatment largely depends on the biological and physicochemical properties of the materials used. In this context, bioactive materials have become increasingly used in endodontic practice due to their biocompatibility and their ability to interact with dental tissues. Objective of the study: To evaluate the properties of bioactive materials used in endodontics by analyzing data reported in the scientific literature in order to demonstrate their role in endodontic treatment. Material and Methods: The study was conducted through the analysis and synthesis of scientific literature from PubMed, Google Scholar and the National Library of the Republic of Moldova. The properties of bioactive materials used in endodontics, such as MTA, Biodentine and BioRoot RCS were analyzed, focusing on characteristics such as biocompatibility, bioactivity, setting time, and antimicrobial effect Results: The synthesis of the scientific literature indicates that bioactive materials used in endodontics present high biocompatibility, demonstrated by the MTT test, with a cell viability of approximately 91.2% for BioRoot RCS and 87.5% for MTA and Biodentine. The literature also reports bioactivity associated with the release of calcium ions and the ability to support mineralization processes. Regarding setting time, Biodentine shows a shorter setting time (12 min) compared with MTA (2–3 hours). The analysis indicates an antimicrobial efficacy of over 90% for these materials. Conclusions: Bioactive materials are an important component of modern endodontics due to their biological and physicochemical properties. The characteristics of these materials, such as their interaction with dental tissues and their biological response, contribute to improving the performance of endodontic treatments. The differences between materials are determined by their composition and specific properties, aspects that may influence their selection in clinical practice. 2026-01-01T00:00:00Z http://repository.usmf.md:80/xmlui/handle/20.500.12710/33206 Invasive potential of cutaneous malignant melanoma Cenușa, Alexandrina; Foca, Ecaterina; Carpenco, Ecaterina; Brînza, Dumitru; David, Valeriu; Șaptefrați, Lilian; Fulga, Veaceslav Introduction. Cutaneous malignant melanoma is the most aggressive skin cancer, with a high mortality rate despite advances in therapy. This study aimed to evaluate the relationship between lymphovascular and perineural invasion and key clinicopathological parameters in superficial spreading melanoma, in order to assess their potential prognostic significance. Materials and methods. A retrospective analysis was conducted on 47 cases of superficial spreading melanoma obtained from the Oncology Institute in Chisinau. All cases were histologically confirmed and reviewed for tumor thickness, Clark level, ulceration, mitotic activity, microsatellitosis, pigmentation, and lymph node involvement. Lymphovascular and perineural invasion were assessed using hematoxylin–eosin staining and, where available, immunohistochemistry. Correlations between invasion patterns and clinicopathologic features were analyzed using Pearson correlation coefficients, with statistical significance set at p < 0.05. Results. Lymphovascular invasion was positive and significantly correlated with tumor thickness (r = 0.54, p< 0.001), Clark level (r = 0.46, p < 0.001), microsatellitosis (r = 0.50, p < 0.001), tumor stage (r = 0.33, p = 0.01), and lymph node involvement (r = 0.29, p = 0.02). A negative correlation was observed with pigmentation (r = –0.26, p = 0.04). Perineural invasion was less frequent, but correlated positively with lymphovascular invasion (r = 0.28, p = 0.03) and showed a trend toward association with amelanotic tumors (r = –0.24, p = 0.05). No significant relationships were found with ulceration or mitotic activity. Conclusions. Lymphovascular invasion represents a significant indicator of aggressive biological behavior in superficial spreading melanoma, closely associated with established prognostic factors. Perineural invasion occurs less frequently, but may further reflect invasive potential, particularly in amelanotic variants. Routine histopathologic assessment of both invasion patterns is recommended to improve prognostic evaluation. 2026-01-01T00:00:00Z http://repository.usmf.md:80/xmlui/handle/20.500.12710/33148 Cells and Tissues Transplantation. Actualities and Perspectives: The Materials of the National Scientific Conference with International Participation, the 4 th edition, Chisinau, March 20-21, 2026. Chișinău 2026-01-01T00:00:00Z http://repository.usmf.md:80/xmlui/handle/20.500.12710/33147 Human collagen-based hydrogel with zinc oxide nanoparticles for articular cartilage engineering Cobzac, Vitalie; Cojocari, Ștefan; Jian, Mariana; Stoian, Alina; Nacu, Ana-Maria; Motelica, Ludmila; Trusca, Roxana; Ficai, Anton; Nacu, Viorel Introduction. Articular cartilage tissue engineering has a long history of using three-dimensional matrices seeded with cells of chondrogenic potential. Recent studies show that chondrogenesis is influenced not only by growth factors, but also by the biochemical microenvironment, including ions such as Zn²⁺ and Cu²⁺. Materials and methods: With approval from the Ethics Committee of Nicolae Testemitanu State University of Medicine and Pharmacy and under a collaboration agreement with the Human Tissue Bank of the Clinical Hospital of Traumatology and Orthopaedics, articular cartilage was collected from a donated femoral head, along with processed umbilical-placental complex. Collagen hydrogels (3 mg/ml) were prepared in 24-well plates containing ZnO NPs (<50 nm; Sigma-Aldrich, USA) at 0 (control), 1, 10, 20, 40, 60, 80, and 100 µg/ml (n=4). Their elastic modulus (EM) was measured using a TX-BLM probe and a TX-700 texture analyzer (Lamy Rheology, France). For viability testing, human chondrocytes (1×105 cells/ml) were cultured on hydrogels containing 0 (control), 10, 50, or 100 µg/ml ZnO NPs. Resazurin (10 µg/ml; Acros TFS, Belgium) was used as the viability reagent, and blanks without cells were prepared for each concentration and control group (n=3). Measurements were performed at 24, 48, and 72 hours after 6 h incubation at 37°C and 5% CO₂. Absorbance was recorded at 570 and 600 nm using a Synergy H3 spectrophotometer (BioTek, USA). Results and Conclusions. ZnO NPs concentration markedly affected the mechanical properties of the hydrogels. Significant differences were found between the control group (470.73 ±38.00 N/m²) and most ZnO-loaded groups. EM was significantly reduced at 60, 80, and 100 µg/ml ZnO (p <0.05), whereas at 1, 10, and 20 µg/ml it was significantly higher than in the control group (p <0.05). No significant difference was observed at 40 µg/ml (p >0.05). The resazurin assay showed a dosedependent effect of ZnO NPs on chondrocyte viability. The highest viability was recorded at 10 µg/ml ZnO, remaining above 70% over three consecutive days, while higher concentrations caused a marked decrease in viability (p < 0.05). Therefore, 10 µg/ml ZnO appears to be the most promising concentration, offering a favorable balance between mechanical strength and cell viability. Acknowledgements: research founded by young researchers project #25.80012.8007.05TC 2026-01-01T00:00:00Z