The Moldovan Medical Journal, Vol. 64, No 2, June 2021

The Moldovan Medical Journal. June 2021, Vol. 64, No 2

Fri, 01 Jan 2021 00:00:00 GMT

The Moldovan Medical Journal. June 2021, Vol. 64, No 2 The Moldovan Medical Journal (MMJ) is a scientific double-blind peer reviewed periodical official edition of the Scientific Medical Association of the Republic of Moldova, designed for specialists in the areas of medicine, dentistry, pharmacy, social medicine and public health. The Scientific Medical Association of Moldova has become the owner and publisher of the journal in 2017. Before that year, since the time of foundation in 1958, the journal was guided by the Ministry of Health. At present, the MMJ publishes independently submitted original researches and review articles, clinical studies and cases, lectures, brief reports and correspondence as well as official papers and editorials in medicine and health sciences. Founder: The Scientific Medical Association of the Republic of Moldova

Professor Constantin Etco – to the 80th anniversary 22.07.1941–19.12.2017

Fri, 01 Jan 2021 00:00:00 GMT

Professor Constantin Etco – to the 80th anniversary 22.07.1941–19.12.2017 Ceban, Emil

Comparative analysis of the skin decellularization methods

Fri, 01 Jan 2021 00:00:00 GMT

Comparative analysis of the skin decellularization methods Macagonova, Olga; Risnic, Doina; Cociug, Adrian; Nacu, Viorel Abstract Background: The extracellular matrix plays an important role in the promoting the tissue regeneration and repair. Decellularization or removal of the cells from the complex mixture of the structural and functional proteins that constitute the extracellular matrix (ECM) can be done by the physical (agitation, sonication, freeze and thaw), chemical (alkaline orchids, ionic detergents, nonionic, tri-n-butyl phosphate (TBP), hypotonic or hypertonic treatments, chelating agents), and enzymatic methods (trypsin or protease inhibitors). However, complications associated with the use of the decellularized skin have been reported, which are widespread and poorly understood. In this synthesis have been included publications, identified by the Google Search engine, National Bibliometric Tool (NBT), Pub Med databases, Web of Science, Springer, Elsevier, Wiley Online Library, Science Direct and Biosience, Biotechnology and Biochemistry. The results of the decellularization were reported in terms of the number of cells remaining in the collagen fibers depending on the duration of exposure to chemical agents. Conclusions: The natural matrix is more widely used than synthetic material, because it has the natural structure and composition of the ECM, it naturally stimulates cell development and allows the incorporation of the growth factors and other proteins increasing cell proliferation.The assessment of the quality of decellularization techniques is done by evaluating the necrosis of the extracellulare matrix, the depletion of the collagen fibers and the remaining amount of genetic material. Laboratory of Tissue Engineering and Cells Culture, Nicolae Testemitanu State University of Medicine and Pharmacy, Human Tissue Bank, Orthopedic and Traumatological Hospital, Chisinau the Republic of Moldova

Cardiomyopathy secondary to Duchenne muscular dystrophy in children

Fri, 01 Jan 2021 00:00:00 GMT

Cardiomyopathy secondary to Duchenne muscular dystrophy in children Rodoman, Iulia; Palii, Ina; Sacara, Victoria; Gladun, Sergiu Abstract. Background: Cardiomyopathy (CM) associated with Duchenne muscular dystrophy (DMD) is a commonly recognized appearance of this neuromuscular disease, significantly increased morbidity and mortality, as well as the necessity for cardiological management. CM in DMD is defined by left ventricular (LV) systolic dysfunction and both atrial and ventricular dysrhythmias and is associated with higher mortality than other cases of pediatric dilated CMs. Notwithstanding the high rate of cardiac involvement, patients are usually asymptomatic despite significant LV dysfunction, because of likely poor mobility that masks the usual heart failure (HF) symptoms. Also, imagistic predictors are provided to be very helpful in defining early LV dysfunction, especially electrocardiogram and cardiac imaging (transthoracic echocardiography, speckle-tracking, cardiac magnetic resonance) are used to detect the onset and progression of dilated cardiomyopathy (DCM) in DMD. Conclusions: As most DMD patients are asymptomatic for a long time of their life, so identifying predictors of HF is crucial to support these patients. Ventricular dysfunction based on the ejection fraction (EF) measurement helps to choose therapy. In the case of early DCM (LVEF≥50%) the great purpose is to prevent ventricular dysfunction incipience with first-line HF therapy with Angiotensin-converting-enzyme inhibitors (ACE-I) or angiotensin receptor blockers (ARBs). Current guidelines recommend the use of conventional HF medication in case of disease progression and DCM with MidRange Reduction of LV EF (40-49%). The therapeutic approach for patients with DCM and severe ventricular dysfunction (<40%) has been studied less profoundly and contemporary guidelines recommend all drugs used for HF treatment. Cardiology Unit, Institute of Mother and Child, Chisinau, the Republic of Moldova, Department of Pediatrics, Nicolae Testemitanu State University of Medicine and Pharmacy, Chisinau, the Republic of Moldova