http://repository.usmf.md:80/xmlui/handle/20.500.12710/27983 The 10th International Medical Congress for Students and Young Doctors, 24-27 April, 2024 Mon, 14 Sep 2026 12:53:57 GMT 2026-09-14T12:53:57Z http://repository.usmf.md:80/xmlui/handle/20.500.12710/29004 Assessment of the cases of postpartum hemorrhage in multiparous women Cemortan, Maria; Bubulici, Cristina; Vicol, Maria-Magdalena; Grajdean, Elena; Scripnic, Gabriela; Manic, Milena Introduction. Postpartum hemorrhage (PPH) is one of the leading obstetric complications, affecting 5-15% births. Being a major factor in maternal mortality and morbidity, PPH causes about 25% of maternal deaths worldwide. Aim of study. The aim of the study was to assess the cases of PPH in multiparous women, admitted to the Tertiary Perinatal Center. Methods and materials. The retrospective study was performed by assessing 81 clinical cases of PPH in multiparous women. Total blood loss in labor or C-section was performed by using graduated vessels, and all the sterile material used was weighted. For continuous variables, the mean values and standard deviation of the mean were calculated; the median (Me) as well as the interquartile range (Q1;Q3) in the case of a distribution of characteristics that differs from the normal. Results. The average age of women was 31.6±5.5 years (Me 32 (28;35.5)), varying in the limits of 20-42 years. The majority of participants delivered for the second time - 38 cases (46.9% (95% CI 33.3-59.9)), however, 30 women (37.0% (95% CI 25.9-48.2)) gave birth for the third time, and 13 women (16.1% (95% CI 8.5-27.4)) had 4th – 9th delivery. In 41 cases (50.6% (95% CI 40.7- 61.7)) a c-section was performed. The mean blood loss in vaginal delivery was 850±308 (Me 800 (600;1050)) mL, varying in the limits of 500– 1600 mL. Compared to the mean blood loss in Csection – 1752±1093 (Me 1500 (1100;1850)) mL, varying in the limits of 1000 – 5250 mL. In the structure of PPH there were assessed 26 cases (32.1% (95% CI 20.9-47.0)) of the placental defect or placenta adherens, 15 cases (18.5% (95% CI 10.3-30.5)) of lacerations of the birth canal, 11 cases (13.6% (95% CI 7.4-23.4)) of uterine atonia, and 2 cases (2.5% (95% CI 0-7.3)) of uterine rupture. Hence, in 46 women (56.8% (95% CI 44.6-69.1)) it was applied conservative management of the cases. However, in 20 cases (24.6% (95% CI 15.0-38.1)) an operative management was applied, from which 7 cases (8.6% (95% CI 3.7-14.7)) hemostatic sutures were applied. In 13 cases (16.0% (95% CI 8.5-27.4)) hysterectomy was performed, from which 9 cases (69.2% (95% CI 31.6-100)) subtotal hysterectomy without annexes was the elective method for definitive hemostasis. Conclusion. PPH is a major obstetric complication, which occurs more frequently in multiparous women, in association with placental pathology and birth canal trauma, explained by overextension of the uterus and coagulation disorders, requiring extensive surgical management. Mon, 01 Jan 2024 00:00:00 GMT http://repository.usmf.md:80/xmlui/handle/20.500.12710/29004 2024-01-01T00:00:00Z http://repository.usmf.md:80/xmlui/handle/20.500.12710/28758 Prospects for obtaining liposomes with standardized plant extract Stahi, Cristina; Yaman, Abdin Introduction. In recent years, scientists have shown an increasing interest in incorporating biologically active compounds extracted from plants into nanoparticle delivery systems. Examples of such delivery systems include liposomes, solid lipid nanoparticles, carbon nanotubes, graphene and many others. The need to develop an advanced phytocompound administration system is based on the fact that most biologically active compounds extracted from plants suffer from having low absorption and undergoing intensive metabolism which limits their efficacy and contributes to their lack of selectivity. Therefore, the incorporation of phytocompound into modern nanoparticle delivery systems would indeed increase their therapeutic efficiency and bioavailability. Aim of study. Firstly, exploring different approaches for generating, analyzing and controlling liposomes reported in the literature, and secondly generating and evaluating liposomes with standardized polyphenolic extract content. Methods and materials. For the literature review, open access scientific articles from databases such as PubMed and Scopus are collected (n=40 articles). With regard to the preparation of liposomes, dry artichoke extract standardized in chlorogenic acid is used, as a polymer, a mixture of Cholesterol: Lecithin: PEG-600 (0,3:1:1) is utilized. Samples are ultrasonicated and dried using a rotary evaporator (IKA VACSTAR). The resulting Liposomes are evaluated under electron microscope (VWR® Binocular Microscope) and their Zeta potential is determined (Malvern Zetasizer Nano ZS). Results. Liposomes possess multiple advantages which allow for the availability of several phytocompounds on the nutraceuticals market. The highest percentage of liposomal phytoconstituents refers to those of phenolic nature with antioxidant properties. Thus, for the research, the standardized extract from artichoke leaves cultivated in the collection of the Practical Scientific Center in the Field of Medicinal Plants of Nicolae Testemitanu SUMPh was used. The liposomes were obtained by the classic method of preparation with the application of the thin film hydration technique, followed by ultrasound, using soy lecithin, PEG 600, cholesterol and methanol as solvent. The obtained phospholipid vesicles with average diameter of 550 nm were observed under the electron microscope, with electric potential of 0.8, determined by means of the dynamic light diffusion method. Conclusion. The research results present important opportunities for capitalizing on natural extractive products through the development of new pharmaceutical forms to ensure the population with quality products and high bioavailability. biologically active compounds extracted from plants into n anoparticle delivery systems. Examples of such delivery systems include liposomes, solid lipid nano particles, carbon nanotubes, graphene and many others. The need to develop an advanced phytocompound administration system is based on the fact that most biologically active compounds extr acted from plants suffer from having low absorption and undergoing intensive metabolism which limits th eir efficacy and contributes to their lack of selectivity. Therefore, the incorporati on of phytocompound into modern nanoparticle delivery systems would indeed increase their therapeutic ef ficiency and bioavailability. Aim of study. Firstly, exploring different approaches for generating, analyzing and controlling liposomes reported in the literature, and secondly generating and evaluating liposomes with standardized polyphenolic extract content. Methods and materials. For the literature review, open access scientific art icles from databases such as PubMed and Scopus are collected (n=40 articles). Wit h regard to the preparation of liposomes, dry artichoke extract standardized in chlorogenic acid is used, as a polymer, a mixture of Cholesterol: Lecithin: PEG-600 (0,3:1:1) is utilized. Sample s are ultrasonicated and dried using a rotary evaporator (IKA VACSTAR). The resulting Liposomes are evaluated under electron microscope (VWR® Binocular Microscope) and their Zeta pote ntial is determined (Malvern Zetasizer Nano ZS). Results. Liposomes possess multiple advantages which allow for the availability of several phytocompounds on the nutraceuticals market. The highest perce ntage of liposomal phytoconstituents refers to those of phenolic nature with a ntioxidant properties. Thus, for the research, the standardized extract from artichoke leav es cultivated in the collection of the Practical Scientific Center in the Field of Medicinal Plants of Nicolae Testemitanu SUMPh was used. The liposomes were obtained by the classic method of preparatio n with the application of the thin film hydration technique, followed by ultrasound, using soy lecithin, PEG 600, cholesterol and methanol as solvent. The obtained phospholipid vesicles with average diameter of 550 nm were observed under the electron microscope, with electric pot ential of 0.8, determined by means of the dynamic light diffusion method. Conclusion. The research results present important opportunities fo r capitalizing on natural extractive products through the development of new pharmace utical forms to ensure the population with quality products and high bioavailability. Universitatea de Stat de Medicină şi Farmacie „Nicolae Testemiţanu”, Chişinău, Republica Moldova Mon, 01 Jan 2024 00:00:00 GMT http://repository.usmf.md:80/xmlui/handle/20.500.12710/28758 2024-01-01T00:00:00Z http://repository.usmf.md:80/xmlui/handle/20.500.12710/28750 Anti-cancer compounds in brassica vegetables Ciurea, Laura Introduction. Cancer is the main cause of mortality and morbidity worldwide. According to the World Cancer Research Fund an estimated 40% of all human cancers are related to diet. Although a large variety of therapeutic approaches have been developed and translated into clinical protocols, the toxic side effects of cancer treatments negatively impact patients, allowing cancer to grow. Cabbage, broccoli, Brussels sprouts, and other members of the g. Brassica have been widely regarded as potentially cancer preventative. A lower risk of colorectal, cervical, and lung cancers was found to be associated with a high intake of cruciferous vegetables. What differentiates Brassicaceae from other plants is the presence of the secondary metabo lites called glucosinolates, recognized for both their role in plant defense and human health. Aim of study. This review aims to examine the roles of Brassicaceae vegetables and their important bioactive metabolites in prevention and treatment of different cancers. Methods and materials. In order to fulfill the purpose of the study the scientific articles (about 60) from the last decade on Google scholar, Science Direct, PubMed databases were searched by following parameters: Brassicaceae nutrients, compounds, phytochemicals; Brassicaceae in health benefits, cancer prevention and treatment and other. Results. The family Brassicaceae genus Brassica consists of the species Brassica oleracea (e.g., broccoli, cabbage, cauliflower, Brussels sprouts, kale, turnips, collards), which are the most frequently consumed vegetables worldwide. In the last couple of decades, growing scientific evidence has suggested that consumption of cruciferous vegetables has a preventive role against a variety of human diseases. It has been demonstrated that the chemopreventive potential of cruciferous vegetables is likely due to glucosinolates and their secondary metabolites (e.g., isothiocyanates (ITCs)). In vitro and in vivo studies have shown that ITCs are able to activate phase II detoxification enzymes (such as quinone reductase and glutathione S-transferase) as well as to disrupt tubulin polymerization, inducing cell cycle arrest and the activation of apoptosis in cancer cells. Additionally, since dietary ITCs are well absorbed and have good bioavailability, these compounds are promising candidates for anti-cancer therapies. Conclusion. The family Brassicaceae signifies to be an outstanding source of health-promoting phytochemicals and nutrients that would pay beneficial dietary importance against certain types of diseases. Numerous epidemiological studies indicate that Brassica vegetables protect humans against cancer. Thus, Brassica metabolites are emerging as new weapons for anti-cancer therapeutics. World Cancer Research Fund an estimated 40% of all human cancers are related to diet. Although a large variety of therapeutic approaches have been devel oped and translated into clinical protocols, the toxic side effects of cancer treatments ne gatively impact patients, allowing cancer to grow. Cabbage, broccoli, Brussels sprouts, and other m embers of the g. Brassica have been widely regarded as potentially cancer preventative. A lower r isk of colorectal, cervical, and lung cancers was found to be associated with a high intake of cruci ferous vegetables. What differentiates Brassicaceae from other plants is the presence of the secondary metabolites called glucosinolates, recognized for both their role in plant defense and human health. Aim of study. This review aims to examine the roles of Brassicaceae ve getables and their important bioactive metabolites in prevention and treatm ent of different cancers. Methods and materials. In order to fulfill the purpose of the study the scientifi c articles (about 60) from the last decade on Google scholar, Science Direct, PubMed databases were searched by following parameters: Brassicaceae nutrients, compounds, phy tochemicals; Brassicaceae in health benefits, cancer prevention and treatment and other. Results. The family Brassicaceae genus Brassica consists of the species Brassica oleracea (e.g., broccoli, cabbage, cauliflower, Brussels sprouts, kale, tur nips, collards), which are the most frequently consumed vegetables worldwide. In the last couple of decades, growing scientific evidence has suggested that consumption of cruciferous vegetabl es has a preventive role against a variety of human diseases. It has been demonstrated t hat the chemopreventive potential of cruciferous vegetables is likely due to glucosinolates and their secondary metabolites (e.g., isothiocyanates (ITCs)). In vitro and in vivo studies have shown that ITCs are able to activate phase II detoxification enzymes (such as quinone reductase an d glutathione S-transferase) as well as to disrupt tubulin polymerization, inducing cell cycle arrest and the activation of apoptosis in cancer cells. Additionally, since dietary ITCs are well absorbed and have good bioavailability, these compounds are promising candidates for anti-cancer therapies. Conclusion. The family Brassicaceae signifies to be an outstanding so urce of health-promoting phytochemicals and nutrients that would pay beneficial dieta ry importance against certain types of diseases. Numerous epidemiological studies indicate that Br assica vegetables protect humans against cancer. Thus, Brassica metabolites are emergin g as new weapons for anti-cancer therapeutics. Universitatea de Stat de Medicină şi Farmacie „Nicolae Testemiţanu”, Chişinău, Republica Moldova Mon, 01 Jan 2024 00:00:00 GMT http://repository.usmf.md:80/xmlui/handle/20.500.12710/28750 2024-01-01T00:00:00Z http://repository.usmf.md:80/xmlui/handle/20.500.12710/28761 Study on clinical cases of atopic dermatitis in children Iepure, Maria Introduction. Allergic contact dermatitis (ACD) was once considered a rarity in children, but recent estimates suggest that it affects 4.4 million children in the United States alone. Currently, there is no cure, but an increasing number of innovative and targeted therapies show promise in gaining control over the disease, even in patients with refractory conditions. This has prompted us to investigate clinical cases of ACD. Aim of study. To evaluate clinical cases of atopic dermatitis in children. Methods and materials. An analysis was conducted on 43 medical records of pediatric atopic dermatitis cases over a 2-year period in outpatient practice. Results. Based on the SCORAD score, it was found that 7% of children exhibited a mild form of progression, 43% had a moderate form, and 50% showed severe progression. Many examined children also suffer from other associated allergic pathologies. Thus, out of the total number of allergic dermatitis patients, 24 children have bronchial asthma (9%) and allergic rhinitis (15%), aligning with existing literature. The most common manifestations observed in children were nonspecific dermatitis on the hands and feet (76%), dry skin (63%), itching (57%), facial erythema (44%), eczema (23%), etc. Current guidelines suggest that sedative antihistamines are favored over non-sedative antihistamines for alleviating allergic itching. We analyzed data from the National Ambulatory Medical Care Survey to compare antihistamine use between dermatologists and nondermatologists. Overall, dermatologists are more inclined to prescribe sedative antihistamines than non-sedative ones compared to non-dermatologists. Patients under the age of 21 were also more likely to receive sedative antihistamines than non-sedative ones. These findings highlight differential prescribing practices for atopic dermatitis among physicians. Conclusion. Providing appropriate antiallergic medication in conjunction with avoiding the detrimental effects of allergic factors will contribute to reducing the incidence of atopic dermatitis morbidity. Among first-generation antihistamines, the most commonly used are clemastine, chlorpheniramine, and cyproheptadine, which are preferably prescribed in acute forms of atopic dermatitis, while second and third-generation antihistamines are typically used in subacute and chronic forms. Universitatea de Stat de Medicină şi Farmacie „Nicolae Testemiţanu”, Chişinău, Republica Moldova Mon, 01 Jan 2024 00:00:00 GMT http://repository.usmf.md:80/xmlui/handle/20.500.12710/28761 2024-01-01T00:00:00Z