Controlled reperfusion and oxidative stress-induced lesions in experimental ovarian torsion/detorsion

Abstract

Ovarian torsion (OT), being a medical emergency, requires being correctly treated in the early stages of the disease, because reperfusion (R) injury may worsen existing lesions, leading to ovarian loss. The purpose of our study was to investigate the advanced oxidation protein products (AOPP) changes in rats’ serum subjected to different OT/detorsion models. Materials and methods. 70 healthy female rats (Rattus albicans), were randomized into 7 groups (n=10): nr. 1: healthy rats – no intervention; nr. 2: the sham group (laparotomy); nr. 3, 4 and 5 – OT for 3 hours and 3 hours ischemia with 1 hour R or 1 hour “on-off “ R; nr. 6 and 7: 3

hours OT and 24 hours R or 24 hours “on-off “ R. The “on- off method”: controlled R by opening and closing the clips

that were applied on the ovarian annexes (on/off) in 10- seconds intervals, for a total of 120 seconds. AOPP level wàs measured in the blood serum samples by Capeillère-Blandin C., et al. method (2004). The results were analyzed by Welch’s ANOVA. Results. A statistically significant higher AOPP level was established in rats’ serum to 24 hours R following OT (mean±SE, 33,23±0,59 μM/L, p=0,001), compared with group nr. 1. The “on-off “ reperfusion method resulted in a decrease in AOPP, 24 hours after “on-off “ reperfusion (26,58±0,56 μM/L, p<0,001). Conclusions: “On-off” method of R minimizes the reperfusion injury in OT.