Эффект влияния Имунофана на надпочечники и щитовидную железу белых крыс при коррекции Метотрексат-индуцированной иммуносупрессии

Abstract

Abstract Background: One of the factors affecting the prevalence of endocrine disease is the immunopathological processes, in particular immunosuppression. The aim was to study the morphometric parameters of the adrenal and thyroid glands of rats at joint application of immunosuppressor “Methotrexate” and immunostimulant “Imunofan” at different periods of observation. The experiment was carried out on 60 white rats of young age (reproductive period). The width of various zones of the cortical substance and the area of adrenocorticocytes of these loci and their nuclei were determined in the adrenal glands. The height and area of thyrocytes (their cytoplasm and nuclei) of follicular epithelium were measured in the thyroid gland. Next, the nuclear-cytoplasmic ratio was calculated. Results: Statistically significant deviations of the studied values were revealed mainly on the 7th, 15th and 30th days after the end of the drug administration: at the same time, in the early stages of observation signs of activation of the adrenal function and depression of the thyroid gland were detected. On the 60th day of observation the studied parameters were restored or were close to those of the control group. Conclusions: The administration of imunofan as corrector of methotrexate-induced immunosuppression allows to neutralize the negative effect of cytostatic drug on morphometric parameters, which allows us to conclude about the close relationship between the endocrine and immune systems.