Analiza imunohistochimică a macrofagelor CD68+ din stroma carcinomului de prostată

Abstract

Abstract Background: Prostate adenocarcinoma often is accompanied by chronic inflammatory processes. Inflammatory cells, in a certain phase of inflammation, achieve a cooperative relationship not only with the tumor cells, but also with the stromal and endothelial cells, in the idea of creating of the suitable microenvironment for the development and progression of cancer. The aim of the study was the quantitative and qualitative characterization, as well as identification of the prognostic value of CD68+ macrophages in prostate adenocarcinoma. Material and methods: Our study included a number of 73 biopsies from patients with a prostate cancer. Specimens were fixed in buffer formalin and paraffin embedded. For immunostaining were used the monoclonal antibody anti-CD68. Macrophages were quantified in the peritumoral and intratumoral areas of the prostate cancer stroma. The results were analysed statistically. Results: The high total density of macrophages was largely determined by the increased number of macrophages in the intratumoral areas. To assess the prognostic value of macrophages in the prostate adenocarcinomas was accomplished a correlation between the total number of CD68+ macrophages and the Gleason score, thus were obtained a statistically significant correlation (p = 0.001). Also, statistically significant correlation was obtained (p = 0.001) between peritumoral CD68+ macrophage density and intratumoral CD34+ vessels. Conclusions: The increase in CD68+ macrophage density and the significant association of their density with the histological grade of the tumor allows us to consider these cells as a prognostic factor in prostate carcinomas.