Abstract:
Abstract
Background: Mammary carcinoma possesses multiple morphological expressions, classified into subtypes based
on histological grade and receptors expression. In spite of various screening and prevention programs implementation its
incidence is still on the top among malignant diseases.
The aim: assessment of EGFR expression based on tumor’s histological type and grade of differentiation and determining of this factor stability within the metastatic process.
Material and methods: there were examined primary tumors and ipsilateral axillary lymph node metastasis, collected from 85 patients with mammary ductal invasive carcinoma of NOS (not otherwise specified) type and 18 patients with
lobular invasive carcinoma, using conventional histological and immunohistochemical (IHC) techniques. With the help
of IHC has been determined the expression of receptor for Epidermal Growth Factor (EGFR).
Results: in ductal invasive carcinoma we obtained weak but statistically significant correlation between histological
grade and age of patients (rs
=0,23, p<0,03). Comparing the values of EGFR expression in both locations, we obtained in 6
cases (15.8%) the expression’s score transfer. All these transfers have been characterized by the loosing of positive pattern
in lymph node microenvironment and were found in both age groups: “before 49 years” and “after 49 years”. In lobular
invasive carcinoma also have been obtained statistically significant correlation between EGFR score of expression and patients age (rs
=0,45, p<0,031). The age correlated with the grade of differentiation, as well (rs
=0,57, p<0,007). Comparing the
values of EGFR in both locations, we determined that the scores of EGFR are statistically different (t=2,12, with an p<0,05).
However the correlation test highlighted strong positive association between EGFR values in primary tumor and
metastasis.
Conclusions: ductal invasive mammary carcinoma of NOS type could in an equal manner EGFR positive or negative.
Histological grade of these tumors positively correlates with the patients’ age. EGFR is unstable during the metastasizing,
the cases of score’s transfer being exclusively characterized by the loosing of this receptor within the lymph node microenvironment. Unstable cases have a low grade of histological differentiation. The tumors with high grade of differentiation
represent negative EGFR pattern. In lobular invasive carcinoma, the most of the tumors are EGFR negative, not only in the
primary tumor, but also in the lymph node metastases. In this type of tumors, the values of EGFR expression depend on
location, however the EGFR’ score in metastasis is directly dependent on its value in the primary tumor
Description:
Catedra de histologie, citologie și embriologie, Laboratorul de morfologie
Universitatea de Stat de Medicină și Farmacie “Nicolae Testemițanu”, Chișinău, Republica Moldova, Conferința Ştiinţifică Internaţională ”Probleme actuale ale morfologiei” dedicată celor 70 de ani de la fondarea Universității de Stat de Medicină și Farmacie „Nicolae Testemiţanu”, Chişinău, 15-16 octombrie 2015