Структурно-функциональные изменения пинеальних клеток епифиза при условиях длительного влияния нитратов и метиленовой сини

Abstract

Abstract Background: the data of this literature mainly devoted to the study of the structure and function of the epiphysis of humans and animals under the conditions of influence on the body of the radioactive and electromagnetic radiation, immobilization of animals, changes of the light hour mode. Leaving poorly learned the questions about the influence on the epiphysis of substances used for the treatment and prevention against poisoning by nitrates, pesticides and other poisonous chemicals. Material and methods: the object for study the status of the epiphysis in postnatal ontogenesis were nonlinear white rats-males of different ages. There were used theoretical, general histologycal, electronmicroscopical methods of the research. Quantitative data were processed by the method of Student – Fisher with the help of computer programs Excel2000, Sigma Plot with the processing of graphic images. Results: With the introduction of methylene blue simultaneously with nitrates in 14-day-old rats on the ultrathin sections of the epiphysis in its different sections can be seen in both the light and dark pineal cells. Under simultaneous action of nitrate and methylene blue in the epiphysis of the 45-day-old experimental rats ultrastructural changes pinealocytes were less pronounced than under the action only of nitrates. Under simultaneous action of nitrate and methylene blue in the epiphysis of the 90-day experimental rats we could see large lipid drops in the bright cells that filled a third of the entire cytoplasm. The mitochondria were of small size and compacted matrix. The core was large, irregularly shaped with diffuse placed the heterochromatin in the nucleoplasm. Dark pinealocyte had slightly enlarged cavity tubules of endoplasmic mesh. We could see the tanks with granular contents in the cytoplasm. Conclusions: the use of methylene blue during the long action of nitrates softens their toxic effect on the pineal cells of the epiphysis and it is a kind of protector of the action of nitrates, reducing the manifestation of the stage of depletion of the general adaptation syndrome.