Дивергенция органогенеза на этапах витального цикла провизорного органа и провизорной стадии формирования кожного регенерата

Abstract

Abstract Background: The main form of the cell organization in a multicellular organism is the organ. Development of the body through the following stages: the formation of embryonic germ tissue differentiation, provisional and definitive organogenesis. According to researchers evolution tissue by means of a mechanism of divergence. In our opinion, this mechanism also applies to the organ level. Material and methods: We studied the morphogenesis of primary human kidney and poultry on 127 human embryos (12-23 Carnegie stage, 25-57 days after fertilization) and 30 human fetuses (9-12 weeks), which were obtained by abortion in women with informed consent, and 268 chicken embryos (cross Hybro PG +, age from 48 hours to 20 days). To study the healing process of the skin defect, we simulated the thermal and chemical burns 100 nonlinear white mice (males weighing 20-30 grams). Thermal burns 2 degrees produced by the apparatus “Tertsik” (own development), chemical burns made using 2,4-dinitrochlorobenzene (rubbed into the skin of mice). All histological material was fixed in 10% formalin, embedded in paraffin. Histological sections stained with hematoxylin and eosin, using PAS-reaction by McManus. We also used immunohistochemical methods (detected Ki-67, CD3 +, CD1α). To speed up the healing of skin defects using the drug gel «Eykovit» (development of the Tyumen State Medical University). Results: identified stages of the life cycle of primary human kidney and birds, their different semantic and functional significance. Also identified and addressed two types of regeneration of the skin (dermal and skin). Details considered cellular migration flows. Conclusions: Divergence is one of the mechanisms of evolution and development of tissues and organs.