Abstract:
Purpose: Analysis of results of allolimbal fragments transportation, conserved by normothermia
within 21-28 days, in post-burn corneal leukoma and recurrent pterygiums.
Methods: Allolimbal fragments transplantation, conserved by normothermia, was performed in 16
eyes, 6 eyes were with post-burn corneal leukoma, and was combined with penetrating
keratoplasty. In 10 eyes with recurrent pterygiums was performed allolimbal transplantation, in
which on the limb area within the dissected body of the pterygium were fitted limbal transplants
and fixed by interrupted sutures. Limbus allotransplantants conservation was performed in the
modified environment DMEM/F12 with glutamine, insulin, dexamethasone, embryonal serum,
HEPES and antibiotic-antimycotics composition in terms of CO2-incubator at 37° C, 95% of
humidity and 5% of CO2, and for 21-28 days.
Results: After keratoplasty in the post-burn corneal leukomas in the early postoperative period the
transplantant epithelialization was observed in the epithelial graft for 7-14 days, due to the limbus
transplants epithelium. Within 6 months after the surgery, corneal transplants has been remained
transparent. After allolimbal transplantation any relapses in patients with recurrent pterygiums was
not observed, patients` observation has been currently continuing. Conservation of limbus
allotranslantants allows significantly increase the number and activity MSC-like limbus cells and
significantly reduce the concentrations of histocompatibility antigens in transplantants, that allows
not to use immunosuppressive therapy in the postoperative period.
Conclusions: Combined performing of penetrating keratoplasty and allolimbal fragments
transplantation, conserved by normothermia within 21-28 days, significantly increases the percent
of corneal transparent retention. Allolimbal fragments transplantation, conserved by normothermia
within 21-28 days, in recurrent pterygium - pathogenetically reasonable treatment method, which
doesn’t require immunosuppressive therapy. Eliminating sectoral limbus deficiency, it creates a
barrier to the growth of the conjunctiva.
