| dc.description.abstract |
Abstract
The purpose of the study was to conduct a
morphopathological evaluation of the efficiency of human
amniotic membrane used in experimental reconstruction of
abdominal wall defects. The study group included 20
Californian rabbits, of both sexes, with the body weight
ranging from 2300 to 2500 g. The abdominal wall defect
was performed surgically under aseptic conditions resecting
a fragment, 10cm x 5cm in size, involving the muscularaponeurotic
layer and parietal peritoneum. Animals
subjected to the intervention of reconstruction of the anterior
abdominal wall defect were divided into 4 groups consisting
of 5 animals, depending on the method of implant
processing and application. Unabsorbable polyester mesh of
Erceokaque type was used in the abdominal wall
reconstruction in the control group. The mesh was fixed to
the abdominal wall layers but it was not covered by skin. In
group 1 there was used the amniotic membrane treated with
0.1% glutaraldehyde protected externally with Stypro
preparation. In group 2 there was used the amniotic
membrane treated with 0.5% formaldehyde. In lot 3 the
cryopreserved amniotic membrane and biological implants
were protected by suturing the skin and subcutaneous layer.
The study results allowed to conclude that the use of the
amniotic membrane as implant, treated with glutaraldehyde
and formalin, does not provide a long-term stability, the
cryopreserved amniotic membrane having some advantages,
namely, implant elasticity and stability of fixation sutures,
as well as a marked reparative-regenerative activity. The
amniotic membrane may be considered a useful temporary
substitute for the peritoneum and a promising non-adherent
adjuvant in reconstructive interventions of the abdominal
wall defects with a viscero-abdominal disproportion. The
obtained results justify the need to continue clinical trials to
evaluate the efficacy and safety of the application of this
biological material. |
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