Quantitative analysis of subgingival microbiota during initial periodontitis

Abstract

ABSTRACT The primary etiology of periodontitis is the presence of specific bacteria in the subgingival plaque biofilm. Thus, it could aid in the risk assessment for early periodontitis. Aim. To make a quantitative analysis of the microbiota of red complex detected in subgingival samples at subjects with initial periodontitis. Materials and methods. The study was conducted on 67 subjects, who were divided in two groups: 54 initial periodontitis subjects and 13 periodontal healthy subjects. Identification of microbiota of red complex was conducted by molecular techniques such as PCR, "Periodont Screen Real-TM‖. Results. Clinically, there was a strong association between GI and CAL to the proportion of red complex in initial periodontitis, which was higher, than in the periodontally healthy subjects. Conclusions. The microbiota analysis of periodontal pathogens in initial periodontitis is a favorable preventive tool in management of periodontal disease initiation and progression.