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- IRMS - Nicolae Testemitanu SUMPh
- REVISTE MEDICALE NEINSTITUȚIONALE
- Revista Farmaceutică a Moldovei
- Revista Farmaceutică a Moldovei 2016
- Revista Farmaceutică a Moldovei Nr. 1-4, 2016
Please use this identifier to cite or link to this item:
http://hdl.handle.net/20.500.12710/16490
Title: | Studiul macro-, microscopic și fitochimic al produsului vegetal din specia Withania somnifera Dunal |
Other Titles: | Macro-, microscopical and phytochemical study of vegetable drugs from sp. Withania somnifera Dunal |
Authors: | Calalb, Tatiana Matveiciuc, Laurita Chiorchina, Nina Cutcovschi, Alina |
Issue Date: | 2016 |
Publisher: | Asociația Farmaciștilor din Republica Moldova |
Citation: | CALALB, Tatiana, MATVEICIUC, Laurita, CHIORCHINA, Nina, CUTCOVSCHI, Alina. Studiul macro-, microscopic și fitochimic al produsului vegetal din specia Withania somnifera Dunal. In: Revista Farmaceutică a Moldovei. 2016, nr. 1-4, p. 19. ISSN 1812-5077. |
Abstract: | Aim of study.
Species Withania somnifera Dunal (fam. Solanaceae)
common name Ashwagandha is spontaneous, herbaceous plants, originary from India and Sri-Lanka. The
content of withanolides of W.radices posses the high
therapeutical effects, that why in nowadays is intensive
cultivated in different regions from different European
and Asian countries. The aim of this work is to study
macro-, microscopical characteristics and qualitative,
quantitative alkaloid analyses of new vegetable drugs (W.
folia and W.herba, obtained from plants grown in the climate conditions of Moldova.
Materials and methods.
The plants of Ashwagandha were multiplicated by
biotechnological methods in vitro in Botany Garden of
Academy of Science of the Republic of Moldova and
grown in greenhouse and open field. The vegetable drugs
(W.radices and W.herba), collected from W.somnifera
plants were studied by microscopical and phytochemical
methods (qualitative reactions for alkaloids identification and dosage of alkaloids by titrimetric method).
Results.
Fragmented vegetable drug of W.radices consists
from specimens of 5-6 cm in length and 1.0 to 2.5 cm
in diameter, cylindrical, gradually tapering down with
a brownish-white surface and pure creamy-white inside
when broken. W.herba includes fragmented stems (outer
surface is hairy, in fracture is white-yellowish), fragmented and entire leaves (simple, exstipulate, petiolate, ovate,
acute up to 10 cm long) separated flowers (complete,
pentamerous, actinomorphic, hermaphrodite), or cymose inflorescences, fragmented and entire fruits (berry
enclosed in gamosepalous calyx). Odour is characteristic,
mucilaginous bitter and acrid taste.
The microscopical indices are: roots – classical primary anatomy structure with oxalic sand in cortex; stem
– epidermis with multicellular denroid trichomes, lacuna
and oxalate sand cells in cortex, cambium, sclerenchymatic lignified fibres and collateral vascular bundles; leaf
- epidermis with multicellular dendroid trichomes and
secretory hairs (uni- or multicellular gland and unicellular stalk), calcium oxalate rosette, fruit – fatty globules
in mesocarp.
The comparative qualitative and quantitative study
of alkaloids in three vegetable drugs were effectuated:
W. folia, W. herba and W. radices, from plants grown in
greenhouse and open field. Qualitative identification of
alkaloids by series of special chemical reactions (Bouchardat reagent, Dragendorff reagent, tannic acid, phosphomolybdic acid, phosphotungstic acid, picric acid, picrolonic acid) demonstrates that alkaloids are present in all
vegetable drugs.
Quantitative study of alkaloids was effectuated by
isolation with chloroform in separated funnel. After drying of alkaloid chloroform extract in acids medium were
determinated the total content of alkaloids by titrimetric
method with solution of sodium hydroxide. The results
denote that the vegetable drugs (W. folia – 1.154%, W.
herba – 1.016%) obtained from the greenhouse plants
contain the higher value of total alkaloids than in vegetable drugs (W. folia – 0.851%, W. herba – 0.784%) from
the open field plants. In both case (plants from greenhouse and in the open field) the highest content of total
alkaloids there is in vegetable drug W. folia (respectively
– 1.154%; 0.851%). Comparison, the highest content of
total alkaloids there is in W. radices (1.415%) than in decreasing W. folia (0.851%) and W. herba (0.784%).
Conclusions.
The specific micro- and microsopical characteristics
to identify the vegetable drugs (W. folia, W. herba and
W. radices) obtained from sp. W.somnifera, grown in the
climate conditions of Moldova were established. The
phytochemical study of 3 vegetable drugs demonstrated
that the main source of alkaloids may be W.radices, and
as alternative – W.folia and W.herb. |
metadata.dc.relation.ispartof: | Revista Farmaceutică a Moldovei: Conferința științifică cu participare internațională „De la design-ul medicamentului la calitate și inofensivitate” în memoria profesorului Filip Babilev „80 ani de la naștere”, 11 Noiembrie 2016, Chișinău, Republica Moldova |
URI: | http://repository.usmf.md/handle/20.500.12710/16490 |
ISSN: | 1812-5077 |
Appears in Collections: | Revista Farmaceutică a Moldovei Nr. 1-4, 2016
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