Methods of bone decalcification and their efficiency

Abstract

The purpose of the paper was to appreciate the optimal decalcification method of the cortical bone for grafting. The objectives of the paper were: determination of the efficiency of decalcification with hydrochloric acid 1M, determination of the efficiency of decalcification with hydrochloric acid 0,5M, determination of the efficiency of decalcification with hydrochloric acid 0,5M being accelerated by electrolysis, determination of the efficiency of decalcification with hydrochloric acid 0,1M being accelerated by electrolysis, determination of the efficiency of decalcification with EDTA 14%. It has been used 73 pieces of bovine compact bone. All bone pieces were devised in five experimental groups (14 pieces for each group) and 3 pieces as control specimens. The experiment duration - was 21 days. Every three days the solutions for demineralization were changed. The presence of Ca2+ ions in the demineralization solution was evaluated every three days, also every three days two specimens from each experimental group were investigated by X-rays methods. After by analytical method was determined the remaining calcium in the specimens.