Qualitative and quantitative determination of proteins in extracts of some medicinal plants

Abstract

Introduction. Global consumption of plant protein is increasing and high-fiber plants have health benefits through valuable phytochemicals. Plant proteins serve as an alternative to animal proteins to meet consumer demand on the one hand and reduce the risk of disease on the other. Material and Methods. We performed qualitative and quantitative determination of proteins in extracts by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and total content of proteins by Bradford assay. Results. Applied methods for the qualitative and quantitative determination of proteins in dry extracts of medicinal plants: Agrimonia eupatoria L., Cichorium intybus L., Galium verum L., and Solidago virgaurea L. confirm that proteins were detected in all dry extracts and were determined the total content of them. Conclusion. For all extracts obtained from aerial parts of: Agrimonia eupatoria, Cichorium intybus, Galium verum and Solidago virgaurea we find a low protein concentration, which implies minimizing allergic reactions and intolerance to the extracts studied.