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Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12710/24238
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dc.contributor.authorJian, Mariana-
dc.contributor.authorCobzac, Vitalie-
dc.contributor.authorVereștiuc, Liliana-
dc.contributor.authorButnaru, Maria-
dc.contributor.authorNacu, Viorel-
dc.date.accessioned2023-05-10T09:05:56Z-
dc.date.available2023-05-10T09:05:56Z-
dc.date.issued2023-
dc.identifier.citationJIAN, Mariana, COBZAC, Vitalie, VEREȘTIUC, Liliana, et al. Scaffolds for bone tissue regeneration. In: Cells and Tissues Transplantation. Actualities and Perspectives: the materials of the national scientific conf. with internat. participation, Chișinău: CEP Medicina. 2023, p. 28. ISBN 978-9975-82-313-5.en_US
dc.identifier.isbn978-9975-82-313-5-
dc.identifier.urihttp://repository.usmf.md/handle/20.500.12710/24238-
dc.description.abstractIntroduction. There are many types of scaffolds prepared in order to regenerate bone tissue defects [2]. In performed research were tested matrices of natural origin. Materials and methods. Collagen sponges were obtained from collagen extracted from the bovine Achilles tendon [3], which were cross-linked with 25% Glutaraldehyde (GA) vapors, and with 25 mM Riboflavin solution under the action of UV-A rays during one hour. Rabbit femoral and iliac wing bones were demineralized in 0.6 M HCl solution. Bone cells (BC) and mesenchymal stem cells (MSC) were isolated from rabbit [4, 5], to perform the MTT assay and to evalulate cellular adhesion on scaffolds with DAPI. The ultrastructure of the scaffolds was analyzed by scanning electron microscopy (SEM). Results. The MTT assay on BC and MSC showed a high cellular activity. Also, seeded cells had a good adhesion and proliferation on the prepared scaffolds at 7 and 14 days of culture. At SEM examination was determined a high porosity of both cross-linked sponges, and thin walls (2-4 µm) with many small cavities in them, through which the pores communicate. However, it is determined that the pores of sponges cross-linked with riboflavin generally have larger sizes (70-220 µm) compared to those cross-linked with GA (50-150 µm) (p <0.01). Also semnificative difference (p <0.01) between pore size was determined in demineralised femoral (25-80 µm) and iliac wing bones (140-520 µm), and with different wall thickness (p <0.01). Conclusions. The obtained scaffolds had a good biocompatibility and a very nonhomogeneous structure.en_US
dc.language.isoenen_US
dc.publisherCEP Medicinaen_US
dc.relation.ispartof„Cells and tissues transplantation. Actualities and perspectives” dedicated to the 10th anniversary of the founding of the Human Tissue and Cells Bank and to the 15th anniversary of the founding of the Laboratory of Tissue Engineering and Cells Culture of Nicolae Testemitanu State University of Medicine and Pharmacy of the Republic of Moldova, March 17-18th 2023, Chisinau, Republic of Moldovaen_US
dc.subjectcollagenen_US
dc.subjectdemineralised boneen_US
dc.subjectscaffoldsen_US
dc.titleScaffolds for bone tissue regenerationen_US
dc.typeOtheren_US
Appears in Collections:The Materials of the National Scientific Conference with International Participation „Cells and tissues transplantation. Actualities and perspectives”

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