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Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12710/27047
Title: Distictive immunohistochemical expression of ang1 in the germinal status of uterine pregnancies disturbed at early term
Authors: David, Valeriu
Petrovici, Virgil
Globa, Tatiana
Carpenco, Ecaterina
Fulga, Veaceslav
Keywords: Ang1;placental implantation;pregnancy;early miscarriage
Issue Date: 2024
Publisher: CEP Medicina
Citation: DAVID, Valeriu, PETROVICI, Virgil, GLOBA, Tatiana, et al. Distictive immunohistochemical expression of ang1 in the germinal status of uterine pregnancies disturbed at early term. In: Cells and Tissues Transplantation. Actualities and Perspectives: the materials of the nat. scientific conf. with internat. particip., the 2nd ed. Chisinau, March 29-30th 2024: [abstracts]. Chişinău: CEP Medicina, 2024, p. 37. ISBN 978-9975-82-366-1.
Abstract: Background. Angiopoietins are part of vasculo- and angiogenesis with an important role in the formation of a functional placental vascular network, capable of supporting the intrauterine development of the embryo and fetus. Disruption of Ang1 expression is associated with the dysregulation of vascular network maturation and stabilization. Aim of the study. Evaluation of the immunohistochemical expression profile of Ang1 in the choriovillary germinal status of patients with early-term pregnancy disturbances. Methods and materials. Tissue samples from 62 patients experiencing early-term pregnancy disturbances were obtained via uterine aspiration at the Mother and Child Institute's Perinatal Center in 2020. Patients were categorized into three groups: early spontaneous abortion (ESA) – 8 cases; missed abortions (MA) – 39 cases; and 15 cases of pregnancy termination for social reasons/desire (TS or TD). Each group was further stratified by gestational age (3-5 weeks, 6-9 weeks, and 10-12 weeks). Methods included hematoxylin-eosin staining, immunohistochemistry assessing anti-Tie1. Immunohistochemical expression was evaluated in cytotrophoblasts, syncytiotrophoblasts, endothelial cells, Hofbauer cells, and stroma, graded from 0 to +3. Statistical analysis involved intensity assessment, Spearman's correlations (rs), and Mann-Whitney (U test). Results. Cytoplasmic immunohistochemical expression ranged from 0 to +2, predominantly negative in controls (68,9%) versus 80% positivity in syncytiotrophoblasts. In the MA group, anti-Ang1 expression rose slightly to +2 (71,7%), peaking at 94,8% in villous syncytiotrophoblasts. ESA group showed significant positivity only in syncytiotrophoblasts (75%), with the rest within 50% positive- negative limits. Statistically significant differences were observed between ESA and control groups in syncytiotrophoblasts (p=0,02) and within MA 10-12 weeks versus MA 3-5 weeks: cytotrophoblasts (p=0,004), vascular endothelium (p=0,02), and cellular stroma (p=0,05); MA 10-12 weeks versus MA 6-9 weeks (p=0,01, p=0,02, and p=0,03). Cytotrophoblasts and vascular endothelium expression negatively correlated with gestational age (GA) (rs=-0,33/0,02 and -0,32/0,02) and age (rs=-0,41/0,01 and -0,36/0,01) in the MA group. In ESA 6-9 weeks, there was a strong positive correlation between age and cytotrophoblasts (rs=0,89; p=0,02), and in ESA total between GA and syncytiotrophoblasts (rs=-0,62/0,05). Conclusion. During placental development, Ang1's angiogenic environment differs in pregnancies disrupted early, leading to a relatively weak angiogenic milieu. Cellular expression varies with gestational term and patient age.
metadata.dc.relation.ispartof: Cells and tissues transplantation. Actualities and perspectives. The 2-nd edition. Chisinau, March 29-30th 2024
URI: http://repository.usmf.md/handle/20.500.12710/27047
ISBN: 978-9975-82-366-1
Appears in Collections:The Materials of the National Scientific Conference with International Participation, the 2nd edition, Chisinau, March 29-30th 2024: [Abstracts]

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