Abstract:
Background: Thiol-disulfide metabolism is essential for normal function of the organism. Thus the interest of the scientists in this area of research
continues to grow.
Material and methods: Copper coordination compounds (CCC), derivatives of thiosemicarbaside (CMD-4, CMJ-33, CMT-67), action on thiol-disulfide
metabolism in the healthy Ratta albicans kidneys were studied. The animals were divided in 6 groups of 7 rats each. The control group included healthy
rats which were injected i/m physiological solution 3 times a week, for 30 days. The rats from groups 2-6 have got 3 times a week, for 30 days, i/m
injections of CCC. The activity of following thiol-disulfide metabolism enzymes in the renal supernatant has been measured: glutathion-reductase (GR),
glutathion-peroxidase (GPO), glutathion-S-transferase (G-S-T), γ-glutamyl transpeptidase (γ-GTP), glutaredoxin (Grx), as well the amount of the protein
SH-groups and of the total glutathione, reduced glutathione (GSH) and oxidized glutathione (GSSG) in renal tissue.
Results: The compounds exhibit different actions: CMT-67 in the dose of 0.1 μM/kg influenced the activity of the glutathione metabolism enzymes –
activated γ-glutamyl transpeptidase (γ-GTP) and glutaredoxine (Grx) and inhibited glutathione reductase (GR), while CMD-4 in doses of 0.1 μM/kg
and 1.0 μM/kg and CMJ-33 in the dose of 1.0 μM/kg significantly diminished the reduced glutathione (GSH) level and increased the amount of the
oxidised one (GSSG).
Conclusions: Selective action of the copper coordination copounds established by this study opens new possibilities of their usage in the therapy of
kidney diseases.
Description:
Laboratory of Biochemistry, Department of Biochemistry and Clinical Biochemistry
Nicolae Testemitanu State University of Medicine and Pharmacy, Chisinau, the Republic of Moldova, Department of Chemistry, State University of Moldova, Chisinau, the Republic of Moldova