Abstract:
Introduction. In the light of the progress achieved in the
area of improving the methods of recombined DNA
technology, today it has become possible to diagnose a
genetic disease, including the Lynch Syndrome as a form of
epithelial colorectal neoplasia (ECRN) at the level of genes
(hMLH1, hMSH2).
Purpose. Analysis of the results of genetic research, at the
molecular level of the hMLH1, hMSH2 genes involved in
triggering the mechanism of tumorogenesis.
Material and methods. During the years 2012-2019, a group
of 55 patients were observed and treated, of which 19
patients were diagnosed with IMS. In the identification of the
genetic associations between the polymorphic DNA spectra
and the clinical manifestations of the studied precancerous
(tumorogenic) diseases, the PCR technique of the isolated
DNA samples from the investigated patients was used.
Results. Following the RT-PCR reaction based on RNA
isolated from the biological material, 30 cases (54.5%) with
negative expression were found, a positive result of grade I
(+) was determined in 14 cases (26, 2%) and grade II (++) in
10 cases (19%). The molecular-genetic origin of NECR was
confirmed and the value of the polymorphic appearance of
the hMLH1, hMSH2 genes, their level and frequency of
expression in tumor tissues was determined.
Conclusions. The RT-PCR technique used in the
experimental study contributes to increasing the efficiency
of the medical-genetic consultation and to the eradication of
colorectal cancer already at the early stages of development.
Description:
State University of Medicine and Pharmacy "Nicolae Testemitanu“, Department of Surgery nr2, Institute of Genetics, Academy of Sciences of Republic of Moldova, Congresul consacrat aniversării a 75-a de la fondarea Universității de Stat de Medicină și Farmacie „Nicolae Testemițanu” din Republica Moldova, Ziua internațională a științei pentru pace și dezvoltare
