Abstract:
Introduction.
Chronic pancreatitis (CP) is a multifactorial pathology, involving
exogenous and endogenous, genetic and non-genetic factors. The
rather high incidence and prevalence of CP in the heterogeneous
population of the RM imposes the need to study the estimated
relative risk of some common risk factors.Purpose
Assessment of the estimated relative risk (OR) in the 95% confidence
interval (CI) of some conventional risk factors for CP.
Material and methods.
The study included 100 patients with CP, mean age-47,02±0,93, m/f-
55/45 and 100 healthy people. Estimated relative risk (OR) in the 95%
confidence interval (CI) of some risk factors for CP [mutations
R122C/PRSS1, R117H/CFTR, N34S/SPINK1, alcohol, smoking,
hypercholesterolemia, hypertriglyceridemia, Body mass index (BMI),
family history (FH) of CP] was determined by using the parametric
modeling system. Gene mutations were evaluated in the Molecular
Genetics Laboratory of the Institute of Genetics, Physiology and Plant
Protection of the Academy of Sciences of Moldova; as a biological
specimen, venous blood was used. The genetic polymorphism was
identified through the polymerase chain reaction and analysis of
enlarged fragment length and restriction fragment length
polymorphism, with the use of the respective primers.Results.
A relatively high estimated risk (OR) has been demonstrated in people who consumed
alcohol-23,22, 95% CI (8,18-71,04), χ2=57,17, p<0,001. Smoking has an OR of 9,41, 95%
Cl (3,91-23,45), χ2=33,27, p<0,001; dyslipidemias: hypercholesterolemia-OR=2,41, 95% CI
(1,18-4,96), χ2=6,00, p<0,05, hypertriglyceridemia-OR=66,00, 95% CI (9,36 -1339,01),
χ2=44,30, p <0,001; FH of CP-OR=3,84, 95% CI (1,99-7,46); R117H/CFTR in heterozygotes-
OR=2,59, 95% Cl (1,30-5,25), χ2=7,53, p<0,01, homozygotes-OR=5,24, 95% Cl (2,04-
13,73), χ2=13,61, p<0,001; R122C/PRSS1 in homozygotes-OR=15,03, 95% Cl (3,08-99,29),
χ2=16,02, p<0,001; N34S/SPINK1 in homozygotes-OR=5,47, 95% Cl (1,93-15,94),
χ2=11,74, p=0,001 (Tab. 1). It is observed that the relative risk for CP is higher in the
presence of the homozygous variant R122C/PRSS1-OR=15.03, exceeding 2,87 times the
relative risk assigned by the R122H/CFTR mutation and 2,75 times by N34S/SPINK1
mutation, the homozygous variant.Conclusions.
There is a high estimated relative risk (OR) in the 95% confidence interval (CI) for CP risk
factors: alcohol, hypertriglyceridemia, smoking, FH of CP, genetic markers, especially in
the homozygous variant, more obviously in the case of mutation R122C/PRSS1.
