Angiogeneza şi mastocitele în leziunile proliferative benigne de prostată

Abstract

Abstract Background: Angiogenesis is essential for tumor growth and metastasis formation. It is assumed that cancers, after an early vasculogenic phase, acquire ability to induce new vessels formation. Such phenomenon is referred to as angiogenic switch. Angiogenic switch depends on the ability to release specific growth factors stimulating blood vessels formation. The source of those factors is both neoplastic cells and various stromal and immune cells, like mast cells. Mast cells (MCs) produce a lot of pro-angiogenic and tissue-remodeling factors. Recent works showed that angiogenic factors are already expressed by the benign lesions. The aim of our study was to investigate the density of CD34-positive blood vessels and number of tryptase-positive mast cells, as well as to establish correlations between microvessel density and mast cells involved in tumor angiogenesis in prostatic benign lesions. Material and methods: Our study included 7 cases of normal prostate, 25 cases of benign prostatic hyperplasia. MCs and the blood vessels were assessed using double immunostaining CD34/MCT. Results: We have shown a total correlation between that two markers in peritumoral areas (p = 0.001, p <0.01 are considered significant) and absence of significant correlation between CD34 and tryptase in intratumoral areas. Conclusions: Our data, as well as, preferential perivascular localization of mast cells and their potential to secrete vascular endothelial growth factor suggests the involvement of mast cells in pathological angiogenesis process from the stage of benign prostate proliferative lesions.