Abstract:
Introduction. The development of degenerative processes relates to the presence of excessive harmful free radicals, which cause damaging oxidative processes within the body.
Various defense mechanisms protect cells from the destructive potential of free radicals.
These include antioxidant enzymes: superoxide dismutase, catalase, glutathione S-transferase, glutathione peroxidase and glutathione reductase. These enzymes play a significant role in reducing oxidative stress, by preventing the spread of harmful free radicals.
Material and methods. The present study used the {N- (prop-2-en-1-yl) -2 - [(pyridin-2-
yl) -methylidene] hydrazine-1-carbothioamide} aquacupper (II) chemical compound and
the MX1 extract – a biological compound, which is a pigment of Myxoxanthophyll carotenoids, obtained from Spirulina platensis biomass at a concentration of 0.214 mg/ml in
80% aqueous solution of ethyl alcohol.
The study also determined both the separate and combined effects of chemical and biological compounds on the spontaneous production of biochemical parameters, which was
carried out in vitro according to the method described by Rîjcova S. et al. with some modifications. To assess the oxidative stress, the malondialdehyde (MDA) concentration and
the advanced oxidation protein products (AOPP) were determined, whereas the antioxidant system was assessed via the identification of the activity of superoxide dismutase
(SOD), total antioxidant (TAA), glutathione-S-transferase (GST), catalase (CT), glutathione
peroxidase (GPX) and glutathione reductase (GR). The blood tests were collected from 10
healthy people aged 25 to 35 years.
Results. The research findings showed that the biological compound under study had positive effects on all the studied parameters, reducing both the MDA, µM/L (p=0.0085) and
AOPP values, µM/L (p=0.018) on the one hand and increasing the potential antioxidant
(SOD, u/c (p=0.0035), CT, µM/L (p=0.0029), TAA, µM/L (p=0.0059), GST, nM/sL
(p=0.024), GPX, nM/sL (p=0.0041) and GR, nM/sL (p=0.0064)) on the other hand. The
tested chemical compound exhibited negative effects, which led to higher MDA, µM/L
(p=0.0085) and AOPP, µM/L (p=0.027) values. However, the chemical compound favored
the antioxidant system (SOD, u/c (p=0.0035), CT, µM/L (p=0.0248), TAA, µM/L
(p=0.0173), GST, nM/sL (p=0.023), GPX, nM/sL (p=0.0365) and GR, nM/sL (p=0.0076)).
While studying the activity results of the tested combined compounds, we found that the
biological compound determines positive effects, particularly on the oxidative stress
markers, though no expected effect potentiation was found.
Conclusions. Based on the obtained research findings regarding the biological compound
with optimal effects on the studied systems, further relevant studies should be carried out.
At the same time, the obtained results require confirmation under in vivo study conditions,
thus not allowing concluding on the quantitative effect of the investigated substances, the
argument being the relatively small number of respondents, determining wide confidence
intervals and being one of the study limitations.
