Determination of the level of biological inflammation markers in patients with anorectal malformations and malformations of the urinary system

Abstract

Aim of the study. To conduct research of the maintenance of cytokines in the serum of blood and urine for children with anorectal malformations in the dynamics of chronic pyelonephritis. Methods. Determined repertoire of the main pro-inflammatory and anti-inflammatory cytokines in urine (IL-1β, IL-6, IL-8, TNF-α, IL-10, IL-1RA) by the ELISA method in 3 points of research: at arrival of the patient in a hospital, prior to antibacterial therapy, in 5-7 days from the beginning of a course of treatment and in 1,5 months after treatment. 54 children with chronic pyelonephritis were examined: 40 patients who had a combination of congenital defects of an urinary system to defects of anorectal area (1 group) and 14 children - without anorectal of defects (the 2nd group). Average age of patients were 4,5±3,6 years old. The group of comparison was made by 20 children with small surgical pathology (umbilical or inguinal hernia) in the preoperative period stratified on age and sex. Results: In the 1st group the reliable increase in concentration of IL- (p<0,007), IL-6 (p<0,003), IL-8 (p<0,003), TNFα (p<0,003) in all three points concerning group of comparison was registered. In the 3rd point of IL-1β (p<0,003), IL-6 (p<0,003), IL-8 (p<0,003) and TNFα (p<0,002) in urine reached the maximum result. In the 2nd group increase of the IL-8 level (p<0,005) concerning group of comparison is noted. The maintenance of TNFα in urine in the 3rd point was much higher, than in the 1st and the 2nd points. Concentration of IL-1β, IL-6, IL-8 in children’s urine of the 1st group was authentically above indicators of the 2nd group. Conclusion. Monitoring of cytokines in urine is a perspective noninvasive method of an assessment of inflammatory process of an uric path at children with anorectal malformation.