Abstract:
Background: Biological materials derived from decellularized tissues could be a good basis for progress in regenerative medicine while maintaining
the main components of the extracellular matrix. A promising scaffold for tissue-engineered is the human amniotic membrane. It is one of the oldest
biomaterials used for scaffolds.
Material and methods: 3 placentas were obtained through Human Tissue Bank. Under sterile condition human amniotic membrane was collected. The
human amniotic membrane was treated with 0.5% of sodium dodecyl sulphate (SDS), 1% Triton for 24 and 5 hours. Amniotic membrane decellularization
was also carried out in combination with ultrasound bath for 20 minutes 3 times. For morphological and structure evaluation of human amniotic membrane
the scanning electron microscopy of native amniotic membrane and histology of decellularized and native amniotic membrane were performed.
Results: The human amniotic membrane decellularization process with 0.5% SDS solution and 1% Triton solution showed that decellularization for 24
hours is too aggressive for human amniotic membrane structure. The decellularization for 5h with 1% Triton solution was incomplete.
Conclusions: The method of decellularization with 0.5% SDS solution is more suitable for amniotic membrane decellularization and can be performed
in only 5 hours. The use of ultrasound bath did not have a significant effect on the obtained results.
