Abstract:
Abstract.
Introduction. Alcohol intoxication is often the cause
of traumatic brain injury. The purpose of the work was to
study the features of lipid metabolism in erythrocytes and
mitochondria of the cerebral cortex cell membranes of rats
in traumatic brain injury on the background of chronic alcohol intoxication.
Materials and methods. The studies were carried out
on 140 male rats of the Wistar line weighing 170-250 g.
Chronic alcohol intoxication was caused by using 15% ethanol as the only source of fluid for 20 days. Reproduction
of traumatic brain injury was carried out by a shock model.
Statistical analysis of data was conducted by using „Primer
Biostatistics 6.0”. The criteria of parametric statistics were
used. The level of p <0.05 was statistically significant.
Results. Chronic alcohol intoxication in rats and inducing of traumatic brain injury separately causes a significant
change of total phospholipids and cholesterol content, and
their molar ratio in the membranes of erythrocytes and mitochondria of the cerebral cortex. Particularly pronounced
were the changes in studied objects with combined pathology, meaning, traumatic brain injury on the background of
chronic alcohol intoxication.
Discoordination of lipid metabolism was unidirectional,
manifested in a significant reduction in the content of total
phospholipids, and increased total cholesterol, which led to
a violation of the molar ratio of total cholesterol/phospholipids in the direction of increasing the coefficient, which is
normally about 1.0.
In subsequent monitoring periods, there was a gradual
restoration of individual fractions of phospholipids, but on
the 30th day of the study, it did not reach the control values. That means that discoordination of the metabolism of
individual phospholipid fractions was so pronounced, that
even on 30th day of arbitrary reproduction did not reach
the initial values, which again emphasizes the severity of
the morphofunctional state of red blood cells membranes’
disturbance.
Conclusions. The revealed features of lipid metabolism
in the membranes of erythrocytes and mitochondria of the
cerebral cortex in traumatic brain injury on the background
of chronic alcohol intoxication are an important component
for understanding the ongoing pathophysiological processes and searching for new effective drugs with targeted action.
