Abstract:
Introduction. Pseudomonas aeruginosastands out as a predominant pathogen causing nosocomial infections, particularly in patients with underlying pathologies or compromised immune systems. The eradication of P. aeruginosahas become increasingly challenging, given its remarkable resistance to antibiotics. Strains of P. aeruginosadeploy both intrinsic and acquired resistance mechanisms, making them formidable against a wide array of antibiotics. Furthermore, adaptive antibiotic resistance in P. aeruginosa, a recently characterized mechanism, involves biofilm-mediated resistance and the formation of multidrug-tolerant persisted cells, contributing to infection relapse. Carbapenem-resistant P. aeruginosa has been identified by the World Health Organization as one of three bacterial species urgently requiring the development of new antibiotics. The overuse of antibiotics in treatment exacerbates the development of multidrug-resistant P. aeruginosastrains, rendering empirical antibiotic therapy ineffective against this microorganism. Aim. Determination of resistance profiles in Pseudomonas aeruginosastrains isolated from clinical bio substrates. Material and methods.The antimicrobial susceptibility profiles of P. aeruginosa isolates were determined using the standard disk diffusion method, following the guidelines outlined by the European Committee on Antimicrobial Susceptibility Testing (EUCAST 2022). An isolate was classified as multidrug-resistant (MDR) if it demonstrated resistance to at least one antimicrobial agent in three or more distinct antibiotic classes. For quality control, the reference strain P. aeruginosa (ATCC 27853) was employed. Results. They analyzed 765 isolates from patients hospitalized in surgical wards. These strains were isolated from various pathological products, including the lower respiratory tract, pus, peritoneal fluid, urine, central venous catheter insertion, blood, feces, bile, and other secretions. P. aeruginosa strains exhibited the following levels of antibiotic resistance: 90.3% to ticarcillin, 78.5% to piperacillin, and 89.6%, respectively 66.8%, to penicillin’scombined with beta-lactamase inhibitors (ticarcillin with clavulanic acid and piperacillin with tazobactam). Resistance to antipseudomonal cephalosporins was observed at 67.6% for ceftazidime and 69.2% for cefepime, while resistance to carbapenems was 68.0% for imipenem and 58.2% for meropenem. Aminoglycoside resistance rates were 52.0% for gentamicin, 67.6% for tobramycin, and 46.4% for amikacin. Notably, 78.5% of strains exhibited cross-resistance to fluoroquinolones (ciprofloxacin, levofloxacin, moxifloxacin, norfloxacin), while resistance to colistin was only 8.4%. A significant majority, 697 strains of P. aeruginosa (91.0%), were classified as multidrug-resistant (MDR). Conclusions.The restricted susceptibility to antimicrobial agents and the frequent emergence of antibiotic resistance during therapy have significantly complicated the treatment of Pseudomonas aeruginosa infections. Consequently, continuous monitoring of drug resistance development in this organism group is of paramount importance. Additionally, the prudent and careful use of antimicrobial agents is imperative to counteract the progression of antimicrobial resistance.
