Abstract:
In prezenta lucrare au fost examinaţi unii factori ce influenţează asupra selectivităţii metodei HPLC. S- au examinat probleme practice de optimizare a realizării de aparat aplicate în analiza cromatografică: alegerea dimensiunilor geometrice ale coloanei şi particulelor de sorbent, repartizarea optimă a modulelor utilajului cromatografic. Au fost studiate efectele precoloanelor la eficienţa rezultantă a sistemului cromatografic. S-a propus o modalitate de excludere a picurilor-artefacte prin selectarea intervalului optim de injectare a probelor. In fine, au fost propuse unele recomandări practice referitor la toate problemele studiate de noi în această lucrare.
The series of the factors which have influence on the HPLC method selectivity have been explored in the given work. The practical problems on optimisation of apparatus realisation of chromatographic analysis are surveyed: a choice of geometrical dimensions of the column and sorbent granules, optimal placement of modules of chromatographic instrumentation. The influence of guard-columns parameters on the resulting efficacy of chromatographic system has been investigated. A method of artefacts exclusion by selection of optimum injection interval was offered. Finally, some practical recommendations on all investigated problems are offered in present word.
