Cultivarea in vitro a limfocitelor recoltate de la pacienţi in stare de sepsis

Abstract

The actual research main idea was to examine the possibility of blood cells cultivation, and especially of lymphocytes from patients in the sepsis stage. Blood was collected from 10 patients in the sepsis stage, from which subsequently has been selected the leucolymphocytar fraction, which later was incubated using Eagle culture, in mono level. At the end of the research the possibility of cultivation of immune-competent blood cells from patients in the sepsis stage was proved.

Scopul studiului dat a fost examinarea posibilităţii cultivării celulelor sangvine, si anume a limfocitelor, la pacienţi in stare de sepsis. S-a colectat sânge venos de la 10 pacienţi in stare de sepsis, din care s-a selectat, fracţia leucolimfocitara sangvină, care ulterior a fost incubată pe mediu de cultură Eagle, in mono strat. In urma studiului s-a demonstrat posibilitatea cultivării celulelor imunocompetente la pacienţi septici.