Abstract:
Our laboratory of mutagenesis is conducting
research on the genotoxicity of chemicals in the battery test systems. Studies carried out in accordance
with the requirements of GLP. One of the metods
used by the laboratory is mammalian in vivo erythrocyte micronucleus test (OECD 474).
We explored 3 samples of active ingredients
of herbicide nicosulfuron from different manufactures. These samples were generic and had various
percentages. Mutagenicity studies examined on CD1
healthy young adult mice, males, which weight was
18-20 g and acclimated to the laboratory conditions
for at least five days.
The test substance was administered as an
aqueous emulsion, once orally. Every samples
studied in three doses 2500, 250, 25 mg kg-1 and
accompanied with positive and negative controls.
The time of exposure was24 hours.
As a result of studies of all of testing samples
of nicosulfuron mutagenic effect was not found.
However, in high concentrations of test chemicals,
it was noted increase of frequency of micronucleus
in polychromatic erythrocytes was not significant in
comparison to the negative control. The relationship
between the frequency of micronuclei, the percentage and impurities is not observed.
Description:
State enterprise «L.I.Medved's research center of preventive toxicology, food and chemical safety, Ministry of health, Prima Conferință științifico-practică națională cu participare internațională ”Siguranţa chimică și toxicologia la confluenţa dintre domenii” 24-25 noiembrie 2016 Chișinău, Republica Moldova
