Utilizarea a di(m-s)-bis{cloro-[1-piridin-2-iletanon-4-metiltiosemicarbazono(1-)]cupru}, în calitate de substanţă cu activitate antimicrobiană faţă de Staphylococcus aureus

Abstract

Background: This work relates to chemistry and medicine, namely to a biologically active coordination compound of copper from the class of thiosemicarbazonates of transition metals, which can be used as substance with antimicrobial activity against Staphylococcus aureus. Material and methods: The antimicrobial activity has been studied in vitro in liquid nutritive media [peptone broth, 2%, pH 7.0] by means of using the method of successive dilutions. The substances were dissolved in DMSO at a concentration of 10 ml, the subsequent dilutions were prepared in peptone broth. The reference strains Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Salmonella abony, Shigella sonnei were used as reference culture. Appreciation of the lowest inhibitory concentration and minimal bactericidal concentrations were performed by the standard method described in the literature. Results: The obtained experimental data show, that this di(m-S)-bis{chloro-[1-pyridine-2-ylethanone-4-methylthiosemicarbazonato(1-)]copper exhibits high antimicrobial activity against selective bacteria of the Staphylococcus aureus, that exceed 80-259 times the same characteristics of structural analogue. Conclusions: The new synthesized coordinative compounds of cooper demonstrate high bacteriostatic and bacteriocide activity against a wide spectrum of both gram-positive as well as gram-negative microorganisms. The experimentally obtained data demonstrate that (di(m-S)-bis{chloro-[1-pyridine2-ylethanone-4-methylthiosemicarbazonato(1-)]copper), show high selective antimicrobial activity against Staphylococcus aureus, which exceeds the analogous features of structural analogue by 80-259 times. Di(m-S)-bis{chloro-[1-pyridine-2-ylethanone-4-methylthiosemicarbazonato(1-)]copper) compounds have bacteriostatic and bacteriocide activity against gram-positive bacteria between the concentrations of 0.00056-0.14mg/ml and gramnegative bacteria between 0.58-75 mg/ml. The identified properties of nominated compounds present interest because of expanding arsenal of antimicrobial remedies and can be used for combating the strains of microorganisms which are resistant to traditional drugs