State of antioxidant system glutathione – glutathione S-transferase in deep fluoridation of tooth enamel in children with high risk of dental caries

Abstract

Background: The high prevalence of dental caries in children determines the relevance of the search of ways to prevent this disease. The goal was a comparative study of the impact of fluor-prevention on the activity of glutathione S-transferase (GST) and content of glutathione of saliva, which play a protective antioxidant and antitoxic role, in children with high risk for dental caries. Material and methods: The study observed 100 children from 7 to 12 years old, 50 children had severe pathology of central nervous system (CNS). Within three years, all children underwent the preventive measures for dental caries, including oral hygiene, complex of vitamins, minerals and antioxidants. Additionally for 60 children was conducted deep fluoridation (DF), a combination of laser therapy with DF, and DF with photodynamic therapy (PDT). Results: The activity of GST, the contents of glutathione and total protein in the saliva were determined by spectrophotometric methods. DF of tooth enamel in healthy children reduced the activity of GST in the saliva in the process of observation, in children with disorders of the nervous system activity of the enzyme increased. Combination of DF with laser therapy decreased the activity of GST in the saliva of children with pathology of CNS, and DF with PDT – it increased. Conclusions: Completing of fluor-preventive complex for caries was effective for both healthy children and children with pathology of CNS.