Abstract:
Introduction: Multiple perioperative factors influence cancer patient evolution and outcome. Microenvironmental factors
activate different gene programs that enable tumor cell to invade, survive and promote drug resistance and metastasis. The effects
of anesthetic drugs on cancer progression is under scrutiny, but published data are controversial and the involved mechanisms
unclear. Tumor development implies PI3K/AKT pathway activation. Akt isoforms (1,2,3) are frequently amplified in various
malignant tumors and associated with malignant cell survival, proliferation and invasion. Their activation is often observed
in human cancers and is associated with decreased survival rate.
Objective: Identification of Akt isoforms activated in sevoflurane exposed breast tumor cells.
Material and methods: Normal breast cells MCF10A (ATCC®) and breast cancer cells MDA-MB-231 (ATCC®) were cultured 2D
(standard adhesive culture plastic plates) and 3D (matrigel). Study groups were exposed to different sevoflurane concentrations
(0.5, 2, 3, 4 mM) compared to control unexposed groups. Unexposed and sevoflurane exposed cells (2D and 3D) were evaluated
by optic microscopy and viability tests. Akt isoforms were assessed by immunofluorescence.
Results: Sevoflurane alters tumor cell proliferation and Akt isoforms expression in a dose-dependent manner. The phenotype
of 3D 2mM sevoflurane exposed cells show an increased migration capacity which indicates increased aggressivity.
Conclusions: Sevoflurane exposure of breast cancer cells influences cell proliferation, phenotype and Akt isoform expression.
Increased sevoflurane concentrations activate different Akt isoforms, putatively related to epithelial-mesenchimal transition
and promote cancer cell invasion, migration and metastasis.
Description:
Grigore T. Popa University of Medicine and Pharmacy, Iași, România, Regional Institute of Oncology, Anaesthesia and Intensive Care Department, Iași, România, Regional Institute of Oncology, Department of Molecular Biology, TRANSCEND, Iași, România,The 5th International Congress of the Society of Anesthesiology and Reanimatology of the Republic of Moldova, 16th Edition of the International Course of Guidelines and Protocols in Anesthesia, Intensive Care and Emergency Medicine, 28th Meeting of the European Society for Computing and Technology in Anesthesia and Intensive Care September 27-29, 2018, Chisinau, the Republic of Moldova