Abstract:
Introduction: Perioperative care of cancer patients is under scrutiny. Among many factors promoting cancer recurrence and
metastasis, high oxygen concentration exposure is underevaluated. While oxygen toxicity is documented in several circumstances,
its implication in tumor cell growth and progression is poorly understood.
Objective: To characterize high oxygen concentration exposure effects on tumor progression using a breast cancer murine model.
Material and methods: A highly aggressive breast tumor cell line 4T1 (ATCC®) was injected in mammary gland in 8 week
old females BALB/c mice. We divided the animals into 3 groups, each including 6 individuals: G1 – tumor bearing mice with
no intervention post inoculation; G2 – primary tumor removal at 2 weeks post inoculation; G3 - primary tumor removal at
2 weeks post inoculation followed by 6 hours of 75% oxygen exposure. In all groups cancer evolution was assessed at 6 weeks
by standard pathomorphological evaluation: specimens from the primary tumor, locally recurrent tumor and target organ
metastasis were assessed by hematoxylin-eosin staining, and digital microscopy.
Results: Surgically removed primary tumors in G3 group had similar characteristics with those in G2 group and previously
described models. At study endpoint, compared with both G2 and G1 groups, G3 animals showed significantly higher tumor
burden: larger local recurrence and more metastasis (larger number and dimensions) in liver and lungs, associated with
significantly enlarged spleen.
Conclusions: Short term (6 hours) high oxygen (75%) concentration exposure results in significantly more aggressive progression
of a 4T1-BALB/c murine breast cancer model.
Description:
Grigore T. Popa University of Medicine and Pharmacy, Iași, România, Regional Institute of Oncology, Anaesthesia and Intensive Care Department, Iași, România, Regional Institute of Oncology, Department of Molecular Biology, TRANSCEND, Iași, România, CEMEX Research Center, Grigore T. Popa University of Medicine and Pharmacy, Iași, România, The 5th International Congress of the Society of Anesthesiology and Reanimatology of the Republic of Moldova, 16th Edition of the International Course of Guidelines and Protocols in Anesthesia, Intensive Care and Emergency Medicine, 28th Meeting of the European Society for Computing and Technology in Anesthesia and Intensive Care September 27-29, 2018, Chisinau, the Republic of Moldova